Abstract

ZnO nanorod structures were deposited on micrometer interdigitated Au electrodes to function as three-dimensional matrixes for the immobilization of antibodies in a capacitive immunosensor format. As a proof of concept, anti-horseradish peroxidase (anti-HRP) antibodies were immobilized on the ZnO nanostructured surface by a crosslinking process. The ZnO nanorod layer allows distribution of antibodies across the entire region probed by the measuring electric field applied to the microelectrodes. This is an alternative approach to the use of more expensive nanometer electrodes necessary in the detection of smaller layers of antibodies. The new micrometer interdigitated capacitive immunosensor was able to discriminate between HRP antigen in buffer; a non-specific antigen in buffer; or buffer alone, as proven by capacitance measurements. Maximum response of the sensor was achieved in the 5-6 kHz frequency range, opening the possibility for a simplified single frequency detection system for direct antigen detection in complex biological samples.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalSensors and Actuators B: Chemical
Volume204
DOIs
Publication statusPublished - 1 Dec 2014

Fingerprint

Immunosensors
antibodies
Nanorods
immobilization
Antibodies
nanorods
antigens
Antigens
Buffers
buffers
micrometers
Electrodes
Capacitance measurement
electrodes
Microelectrodes
Horseradish Peroxidase
crosslinking
Crosslinking
format
frequency ranges

Keywords

  • Biosensor
  • Capacitive immunosensor
  • Impedance spectroscopy
  • Interdigitated electrodes
  • ZnO nanorods

Cite this

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title = "ZnO nanorods as immobilization layers for interdigitated capacitive immunosensors",
abstract = "ZnO nanorod structures were deposited on micrometer interdigitated Au electrodes to function as three-dimensional matrixes for the immobilization of antibodies in a capacitive immunosensor format. As a proof of concept, anti-horseradish peroxidase (anti-HRP) antibodies were immobilized on the ZnO nanostructured surface by a crosslinking process. The ZnO nanorod layer allows distribution of antibodies across the entire region probed by the measuring electric field applied to the microelectrodes. This is an alternative approach to the use of more expensive nanometer electrodes necessary in the detection of smaller layers of antibodies. The new micrometer interdigitated capacitive immunosensor was able to discriminate between HRP antigen in buffer; a non-specific antigen in buffer; or buffer alone, as proven by capacitance measurements. Maximum response of the sensor was achieved in the 5-6 kHz frequency range, opening the possibility for a simplified single frequency detection system for direct antigen detection in complex biological samples.",
keywords = "Biosensor, Capacitive immunosensor, Impedance spectroscopy, Interdigitated electrodes, ZnO nanorods",
author = "P. Sanguino and Tiago Monteiro and Bhattacharyya, {S. R.} and Dias, {C. J.} and Rui Igreja and Ricardo Franco",
note = "This work was supported by Funda{\cc}{\~a}o para a Ci{\^e}ncia e a Tecnologia, Portugal (Grants PEstC/EQB/LA0006/2013 to PS and RF; PTDC/CTM-NAN/112241/2009 to RF; post-doc grant SFRH/BPD/70803/2010 to PS; post-doc grant SFRH/BPD/66773/2009 to SRB; and the COMPETE Program).",
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AU - Sanguino, P.

AU - Monteiro, Tiago

AU - Bhattacharyya, S. R.

AU - Dias, C. J.

AU - Igreja, Rui

AU - Franco, Ricardo

N1 - This work was supported by Fundação para a Ciência e a Tecnologia, Portugal (Grants PEstC/EQB/LA0006/2013 to PS and RF; PTDC/CTM-NAN/112241/2009 to RF; post-doc grant SFRH/BPD/70803/2010 to PS; post-doc grant SFRH/BPD/66773/2009 to SRB; and the COMPETE Program).

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N2 - ZnO nanorod structures were deposited on micrometer interdigitated Au electrodes to function as three-dimensional matrixes for the immobilization of antibodies in a capacitive immunosensor format. As a proof of concept, anti-horseradish peroxidase (anti-HRP) antibodies were immobilized on the ZnO nanostructured surface by a crosslinking process. The ZnO nanorod layer allows distribution of antibodies across the entire region probed by the measuring electric field applied to the microelectrodes. This is an alternative approach to the use of more expensive nanometer electrodes necessary in the detection of smaller layers of antibodies. The new micrometer interdigitated capacitive immunosensor was able to discriminate between HRP antigen in buffer; a non-specific antigen in buffer; or buffer alone, as proven by capacitance measurements. Maximum response of the sensor was achieved in the 5-6 kHz frequency range, opening the possibility for a simplified single frequency detection system for direct antigen detection in complex biological samples.

AB - ZnO nanorod structures were deposited on micrometer interdigitated Au electrodes to function as three-dimensional matrixes for the immobilization of antibodies in a capacitive immunosensor format. As a proof of concept, anti-horseradish peroxidase (anti-HRP) antibodies were immobilized on the ZnO nanostructured surface by a crosslinking process. The ZnO nanorod layer allows distribution of antibodies across the entire region probed by the measuring electric field applied to the microelectrodes. This is an alternative approach to the use of more expensive nanometer electrodes necessary in the detection of smaller layers of antibodies. The new micrometer interdigitated capacitive immunosensor was able to discriminate between HRP antigen in buffer; a non-specific antigen in buffer; or buffer alone, as proven by capacitance measurements. Maximum response of the sensor was achieved in the 5-6 kHz frequency range, opening the possibility for a simplified single frequency detection system for direct antigen detection in complex biological samples.

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