Zika detection: comparison of methodologies

Colombo, Tatiana Elias, Ana Carolina Bernardes Terzian, Araujo-Jr, João P., R Parreira, Cabrera, Eliana Márcia Sotello, Santos, Izalco Nuremberg Penha Dos, Reis, Andréia Francesli Negri, Costa, Fabiana Rodrigues, Cruz, Lilian Elisa Arão Antônio, Rombola, Patrícia Lopes, Maurício Lacerda Nogueira

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Many countries in the Americas have detected local transmission of multiple arboviruses that cause febrile illnesses. Therefore, laboratory testing has become an important tool for confirming the etiology of these diseases. The present study aimed to compare the sensitivity and specificity of three different Zika virus detection assays. One hundred serum samples from patients presenting with acute febrile symptoms were tested using a previously reported TaqMan® RT-qPCR assay. We used a SYBR® Green RT-qPCR and a conventional PCR methodologies to compare the results. Of the samples that were determined to be negative by the TaqMan® RT-qPCR assay, 100% (Kappa=0.670) were also found to be negative by SYBR® Green RT-qPCR based on Tm comparison; however, 14% (Kappa=0.035) were found to be positive by conventional PCR followed by agarose gel electrophoresis. The differences between the ZIKV strains circulating worldwide and the low viremia period can compromise diagnostic accuracy and thereby the accuracy of outbreak data. Therefore, improved assays are required to improve the diagnosis and surveillance of arbovirus.
Original languageEnglish
Pages (from-to)144-147
Number of pages4
JournalBrazilian Journal Of Microbiology
VolumeVol. 49
Issue numbern.º 1
Publication statusPublished - Jan 2018


  • Detection
  • Sensitivity
  • Specificity
  • Zika virus


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