Xenopus Pkdcc1 and Pkdcc2 are two new tyrosine kinases involved in the regulation of JNK dependent Wnt/PCP signaling pathway

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Abstract

Protein Kinase Domain Containing, Cytoplasmic (PKDCC) is a protein kinase which has been implicated in longitudinal bone growth through regulation of chondrocytes formation. Nevertheless, the mechanism by which this occurs remains unknown. Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are both involved on blastopore and neural tube closure during gastrula and neurula stages, respectively. In vertebrates, tissue polarity and cell movement observed during gastrulation and neural tube closure are controlled by Wnt/Planar Cell Polarity (PCP) molecular pathway. Our results showed that Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane. But surprisingly, they revealed different roles in the induction of a luciferase reporter under the control of Atf2 promoter. While Pkdcc1 induces Atf2 expression, Pkdcc2 does not, and furthermore inhibits its normal induction by Wnt11 and Wnt5a. Altogether our data show, for the first time, that members of the PKDCC family are involved in the regulation of JNK dependent Wnt/PCP signaling pathway.
Original languageEnglish
Article numbere0135504
Pages (from-to)Online
JournalPLoS ONE
Volume10
Issue number8
DOIs
Publication statusPublished - 13 Aug 2015

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Cell Polarity
Xenopus
mitogen-activated protein kinase
protein kinases
Protein-Tyrosine Kinases
Protein Kinases
tyrosine
phosphotransferases (kinases)
Gastrula
Neural Tube
Molecular polarity
Gastrulation
chondrocytes
Bone Development
Xenopus laevis
luciferase
Cell membranes
Chondrocytes
Luciferases
cell movement

Keywords

  • Multidisciplinary Sciences

Cite this

@article{6d8c6677d1d24aa6ba2b6f392e05d210,
title = "Xenopus Pkdcc1 and Pkdcc2 are two new tyrosine kinases involved in the regulation of JNK dependent Wnt/PCP signaling pathway",
abstract = "Protein Kinase Domain Containing, Cytoplasmic (PKDCC) is a protein kinase which has been implicated in longitudinal bone growth through regulation of chondrocytes formation. Nevertheless, the mechanism by which this occurs remains unknown. Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are both involved on blastopore and neural tube closure during gastrula and neurula stages, respectively. In vertebrates, tissue polarity and cell movement observed during gastrulation and neural tube closure are controlled by Wnt/Planar Cell Polarity (PCP) molecular pathway. Our results showed that Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane. But surprisingly, they revealed different roles in the induction of a luciferase reporter under the control of Atf2 promoter. While Pkdcc1 induces Atf2 expression, Pkdcc2 does not, and furthermore inhibits its normal induction by Wnt11 and Wnt5a. Altogether our data show, for the first time, that members of the PKDCC family are involved in the regulation of JNK dependent Wnt/PCP signaling pathway.",
keywords = "ANTERIOR PRIMITIVE ENDODERM, VERTEBRATE GASTRULATION, WNT, NEURAL-TUBE CLOSURE, APICAL CONSTRICTION, SECRETED FACTOR, PROTEIN, PLANAR CELL POLARITY, CONVERGENT EXTENSION, PCP PATHWAY, Multidisciplinary Sciences",
author = "Jos{\'e} Belo and Ramalho, {Jos{\'e} da Silva} and In{\'a}cio, {Jos{\'e} Manuel Caf{\'e}}",
note = "info:eu-repo/grantAgreement/FCT/3599-PPCDT/69912/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/132896/PT# This work was supported by research grants from the Fundacao Ciencia e Tecnologia - IP (http://www.fct.pt/index.phtml.pt), and from IBB/CBME, LA to J. A. Belo in the frame of Projects ref degrees PTDC/BIA-BCM/69912/2006 and Pest-OE/EQB/LA0023/2013. M. Vitorino, A. C. Silva and J. M. Inacio are recipients of FCT post-doc fellowships.",
year = "2015",
month = "8",
day = "13",
doi = "10.1371/journal.pone.0135504",
language = "English",
volume = "10",
pages = "Online",
journal = "PlosOne",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "8",

}

TY - JOUR

T1 - Xenopus Pkdcc1 and Pkdcc2 are two new tyrosine kinases involved in the regulation of JNK dependent Wnt/PCP signaling pathway

AU - Belo, José

AU - Ramalho, José da Silva

AU - Inácio, José Manuel Café

N1 - info:eu-repo/grantAgreement/FCT/3599-PPCDT/69912/PT# info:eu-repo/grantAgreement/FCT/3599-PPCDT/132896/PT# This work was supported by research grants from the Fundacao Ciencia e Tecnologia - IP (http://www.fct.pt/index.phtml.pt), and from IBB/CBME, LA to J. A. Belo in the frame of Projects ref degrees PTDC/BIA-BCM/69912/2006 and Pest-OE/EQB/LA0023/2013. M. Vitorino, A. C. Silva and J. M. Inacio are recipients of FCT post-doc fellowships.

PY - 2015/8/13

Y1 - 2015/8/13

N2 - Protein Kinase Domain Containing, Cytoplasmic (PKDCC) is a protein kinase which has been implicated in longitudinal bone growth through regulation of chondrocytes formation. Nevertheless, the mechanism by which this occurs remains unknown. Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are both involved on blastopore and neural tube closure during gastrula and neurula stages, respectively. In vertebrates, tissue polarity and cell movement observed during gastrulation and neural tube closure are controlled by Wnt/Planar Cell Polarity (PCP) molecular pathway. Our results showed that Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane. But surprisingly, they revealed different roles in the induction of a luciferase reporter under the control of Atf2 promoter. While Pkdcc1 induces Atf2 expression, Pkdcc2 does not, and furthermore inhibits its normal induction by Wnt11 and Wnt5a. Altogether our data show, for the first time, that members of the PKDCC family are involved in the regulation of JNK dependent Wnt/PCP signaling pathway.

AB - Protein Kinase Domain Containing, Cytoplasmic (PKDCC) is a protein kinase which has been implicated in longitudinal bone growth through regulation of chondrocytes formation. Nevertheless, the mechanism by which this occurs remains unknown. Here, we identified two new members of the PKDCC family, Pkdcc1 and Pkdcc2 from Xenopus laevis. Interestingly, our knockdown experiments revealed that these two proteins are both involved on blastopore and neural tube closure during gastrula and neurula stages, respectively. In vertebrates, tissue polarity and cell movement observed during gastrulation and neural tube closure are controlled by Wnt/Planar Cell Polarity (PCP) molecular pathway. Our results showed that Pkdcc1 and Pkdcc2 promote the recruitment of Dvl to the plasma membrane. But surprisingly, they revealed different roles in the induction of a luciferase reporter under the control of Atf2 promoter. While Pkdcc1 induces Atf2 expression, Pkdcc2 does not, and furthermore inhibits its normal induction by Wnt11 and Wnt5a. Altogether our data show, for the first time, that members of the PKDCC family are involved in the regulation of JNK dependent Wnt/PCP signaling pathway.

KW - ANTERIOR PRIMITIVE ENDODERM

KW - VERTEBRATE GASTRULATION

KW - WNT

KW - NEURAL-TUBE CLOSURE

KW - APICAL CONSTRICTION

KW - SECRETED FACTOR

KW - PROTEIN

KW - PLANAR CELL POLARITY

KW - CONVERGENT EXTENSION

KW - PCP PATHWAY

KW - Multidisciplinary Sciences

U2 - 10.1371/journal.pone.0135504

DO - 10.1371/journal.pone.0135504

M3 - Article

VL - 10

SP - Online

JO - PlosOne

JF - PlosOne

SN - 1932-6203

IS - 8

M1 - e0135504

ER -