Xenodiagnosis in four domestic cats naturally infected by Leishmania infantum

Geovanna Vioti, Mariana Dantas da Silva, Fredy Galvis-Ovallos, Maria Luana Alves, Diogo Tiago da Silva, João Augusto Franco Leonel, Nuno Wolfgang Balbini Pereira, Julia Cristina Benassi, Júlio Cesar Pereira Spada, Carla Maia, Eunice Aparecida Bianchi Galati, Wilma Aparecida Starke-Buzetti, Trícia Maria Ferreira de Sousa Oliveira

Research output: Contribution to journalArticlepeer-review

Abstract

Leishmaniasis is a neglected tropical disease that continues to pose a serious public health problem. Albeit dogs have long been held as the major reservoirs of Leishmania infantum, the involvement of domestic cats in the zoonotic cycle of visceral leishmaniasis has gained prominence. Here, 240 cats were evaluated by clinical signs and haematological/biochemical changes compatible with leishmaniasis and were diagnosed by serological, molecular, and parasitological techniques. Thus, four cats naturally infected by L. infantum were submitted to xenodiagnosis. A total of 203 females of Lutzomyia longipalpis were subjected to feeding on four cats, with all females completing the blood meal. Parasitological and molecular assays were carried out to evaluate the presence of L. infantum in the sand flies' midgut. Promastigotes were observed in 10 females (6.5%) that fed on one cat, and L. infantum DNA was detected in 17 (8.4%) females which fed on two cats. Our results strengthen the evidence that naturally infected cats are capable of transmitting L. infantum to sand flies. This article is protected by copyright. All rights reserved.

Original languageEnglish
Number of pages9
JournalTransboundary and Emerging Diseases
DOIs
Publication statusE-pub ahead of print - 6 Jul 2021

Keywords

  • Feline leishmaniasis, Leishmania infantum, Lutzomyia longipalpis, visceral leishmaniasis

Fingerprint

Dive into the research topics of 'Xenodiagnosis in four domestic cats naturally infected by Leishmania infantum'. Together they form a unique fingerprint.

Cite this