The support that radiological examinations provide for medical diagnosis is essential. Over time, an increase in the performance of these exams has been observed and, consequently, an increase in people exposed to X-rays. For biological reasons, women are most sensitive to X-rays. It becomes important to analyse their health literacy levels, safeguarding radiological protection in the future. An online questionnaire was developed, and disseminated on the digital platforms of three associations, aimed at women aged between 18 and 57. A sample of 502 women was obtained. It was possible to observe gaps in health literacy, namely in dose, as well as in the X-rays presence in some exams, such as “Mammography” and “Bone Density”. Variables such as “Age” and “Academic Qualification” are shown to directly influence the literacy levels presented. On the other hand, the information provided by the Healthcare Professionals did not reveal to exert any influence on the levels of women’s radiological knowledge. Betting on greater and better communication in radiology for this target audience, considering the sociodemographic variables of each one, will promote greater interest and participation of Health Users in the process, helping to increase radiological protection and safety.