TY - JOUR
T1 - Whole-genome analysis uncovers loss of blaZ associated with carriage isolates belonging to methicillin-resistant Staphylococcus aureus (MRSA) clone ST5-VI in Cape Verde
AU - Wysocka, Magdalena
AU - Monteiro, Tamar
AU - de Pina, Carine
AU - Gonçalves, Deisy
AU - de Pina, Sandrine
AU - Ludgero-Correia, Antonio
AU - Moreno, Joao
AU - Zamudio, Roxana
AU - Almebairik, Nada
AU - Gray, Laura J.
AU - Pareek, Manish
AU - Jenkins, David R.
AU - De Sousa, Marta Aires
AU - De Lencastre, Herminia
AU - Beleza, Sandra
AU - Araujo, Isabel I.
AU - Conceição, Teresa
AU - Oggioni, Marco R.
N1 - Funding Information:
This work was funded by a grant from the Academy of Medical Sciences Global Challenges Research Fund Networking Grant Scheme [GCRFNG\100338 to IIM and SB]; an IRDF travel grant of the University of Leicester to MRO; project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020-Programa Operacional Competitividade e Internacionaliza??o (POCI); national funds through Funda??o para a Ci?ncia e a Tecnologia (FCT), Portugal; ONEIDA project LISBOA-01-0145-FEDER-016417 co-funded by FEEI-?Fundos Europeus Estruturais e de Investimento? from ?Programa Operacional Regional Lisboa 2020?; and national funds from FCT, Portugal. MP is supported by the National Institute for Health Research [NIHR Post-Doctoral Fellowship, Dr Manish Pareek, PDF-2015-08-102]. Oral informed consent from each participant or from their guardians in the case of children was obtained at the time of sampling.
Funding Information:
This work was funded by a grant from the Academy of Medical Sciences Global Challenges Research Fund Networking Grant Scheme [GCRFNG\100338 to IIM and SB]; an IRDF travel grant of the University of Leicester to MRO; project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE2020-Programa Operacional Competitividade e Internacionalização (POCI); national funds through Fundação para a Ciência e a Tecnologia (FCT), Portugal; ONEIDA project LISBOA-01-0145-FEDER-016417 co-funded by FEEI-‘Fundos Europeus Estruturais e de Investimento’ from ‘Programa Operacional Regional Lisboa 2020’; and national funds from FCT, Portugal. MP is supported by the National Institute for Health Research [NIHR Post-Doctoral Fellowship, Dr Manish Pareek, PDF-2015-08-102].
Publisher Copyright:
© 2021 The Authors
PY - 2021/9
Y1 - 2021/9
N2 - Objectives: Surveillance studies for Staphylococcus aureus carriage are a primary tool to survey the prevalence of methicillin-resistant S. aureus (MRSA) in the general population, patients and healthcare workers. We have previously reported S. aureus carriage in various African countries, including Cape Verde. Methods: Whole-genome sequences of 106 S. aureus isolates from Cape Verde were determined. Results: Staphylococcus aureus carriage isolates in Cape Verde show high genetic variability, with the detection of 27 sequence types (STs) and three primary genetic clusters associated with ST152, ST15 and ST5. One transmission event with less than eight core-genome single nucleotide polymorphisms (cgSNP) differences was detected among the ST5-VI MRSA lineage. Genetic analysis confirmed the phenotypic resistance and allowed the identification of six independent events of plasmid or transposon loss associated with the deletion of blaZ in nine isolates. In the four ST5 MRSA isolates, loss of the blaZ plasmid coincided with the acquisition of SCCmec type VI and an unusual penicillin phenotype with a minimum inhibitory concentration (MIC) at the breakpoint, indicating an adaptation trend in this endemic lineage. Similar events of blaZ plasmid loss, with concomitant acquisition SCCmec elements, were detected among ST5 isolates from different geographical origins. Conclusion: Overall, the genome data allowed to place isolates in a phylogenetic context and to identify different blaZ gene deletions associated with plasmid or transposon loss. Genomic analysis unveiled adaptation and evolution trends, namely among emerging MRSA lineages in the country, which deserve additional consideration in the design of future infection control protocols.
AB - Objectives: Surveillance studies for Staphylococcus aureus carriage are a primary tool to survey the prevalence of methicillin-resistant S. aureus (MRSA) in the general population, patients and healthcare workers. We have previously reported S. aureus carriage in various African countries, including Cape Verde. Methods: Whole-genome sequences of 106 S. aureus isolates from Cape Verde were determined. Results: Staphylococcus aureus carriage isolates in Cape Verde show high genetic variability, with the detection of 27 sequence types (STs) and three primary genetic clusters associated with ST152, ST15 and ST5. One transmission event with less than eight core-genome single nucleotide polymorphisms (cgSNP) differences was detected among the ST5-VI MRSA lineage. Genetic analysis confirmed the phenotypic resistance and allowed the identification of six independent events of plasmid or transposon loss associated with the deletion of blaZ in nine isolates. In the four ST5 MRSA isolates, loss of the blaZ plasmid coincided with the acquisition of SCCmec type VI and an unusual penicillin phenotype with a minimum inhibitory concentration (MIC) at the breakpoint, indicating an adaptation trend in this endemic lineage. Similar events of blaZ plasmid loss, with concomitant acquisition SCCmec elements, were detected among ST5 isolates from different geographical origins. Conclusion: Overall, the genome data allowed to place isolates in a phylogenetic context and to identify different blaZ gene deletions associated with plasmid or transposon loss. Genomic analysis unveiled adaptation and evolution trends, namely among emerging MRSA lineages in the country, which deserve additional consideration in the design of future infection control protocols.
KW - Antibiotic resistance
KW - Cape Verde
KW - Genomic epidemiology
KW - Methicillin-resistant
KW - MRSA
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=85108427734&partnerID=8YFLogxK
U2 - 10.1016/j.jgar.2021.04.018
DO - 10.1016/j.jgar.2021.04.018
M3 - Article
C2 - 34052522
AN - SCOPUS:85108427734
SN - 2213-7165
VL - 26
SP - 77
EP - 83
JO - Journal of Global Antimicrobial Resistance
JF - Journal of Global Antimicrobial Resistance
ER -