West Nile virus in Southern Portugal, 2004

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52 Citations (Scopus)


West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.

Original languageEnglish
Pages (from-to)410-413
Number of pages4
JournalVector-Borne and Zoonotic Diseases
Issue number4
Publication statusPublished - 1 Dec 2005


  • Culex mosquitoes
  • Flavivirus
  • NS5 gene
  • Southern Portugal
  • West Nile virus


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