West Nile virus in Southern Portugal, 2004

Aida Esteves; A. Paulo G. Almeida; Rui Pedro Galão; Ricardo Parreira; João Piedade; João C. Rodrigues; Carla A. Sousa; M. Teresa Novo

doi:10.1089/vbz.2005.5.410

West Nile virus in Southern Portugal, 2004

Aida Esteves, A. Paulo G. Almeida, Rui Pedro Galão, Ricardo Parreira, João Piedade, João C. Rodrigues, Carla A. Sousa, M. Teresa Novo

Instituto de Higiene e Medicina Tropical (IHMT)

Research output: Contribution to journal › Article › peer-review

52 Citations (Scopus)

Abstract

West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.

Original language	English
Pages (from-to)	410-413
Number of pages	4
Journal	Vector-Borne and Zoonotic Diseases
Volume	5
Issue number	4
DOIs	https://doi.org/10.1089/vbz.2005.5.410
Publication status	Published - 1 Dec 2005

Keywords

Culex mosquitoes
Flavivirus
NS5 gene
Southern Portugal
West Nile virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/vbz.2005.5.410

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title = "West Nile virus in Southern Portugal, 2004",

abstract = "West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.",

keywords = "Culex mosquitoes, Flavivirus, NS5 gene, Southern Portugal, West Nile virus",

author = "Aida Esteves and Almeida, {A. Paulo G.} and Gal{\~a}o, {Rui Pedro} and Ricardo Parreira and Jo{\~a}o Piedade and Rodrigues, {Jo{\~a}o C.} and Sousa, {Carla A.} and Novo, {M. Teresa}",

year = "2005",

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doi = "10.1089/vbz.2005.5.410",

language = "English",

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journal = "Vector-Borne and Zoonotic Diseases",

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T1 - West Nile virus in Southern Portugal, 2004

AU - Esteves, Aida

AU - Almeida, A. Paulo G.

AU - Galão, Rui Pedro

AU - Parreira, Ricardo

AU - Piedade, João

AU - Rodrigues, João C.

AU - Sousa, Carla A.

AU - Novo, M. Teresa

PY - 2005/12/1

Y1 - 2005/12/1

N2 - West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.

AB - West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.

KW - Culex mosquitoes

KW - Flavivirus

KW - NS5 gene

KW - Southern Portugal

KW - West Nile virus

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JF - Vector-Borne and Zoonotic Diseases

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ER -

West Nile virus in Southern Portugal, 2004

Abstract

Keywords

UN SDGs

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