Virus Production for Clinical Gene Therapy

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Gene therapy is becoming increasingly relevant for the treatment of prominent human diseases. Viral vectors are currently used in more than 50% of the gene therapy clinical trials, most of them aimed at cancer diseases. Clearly, the increasing needs of high-quality viral preparations required the elimination of process bottlenecks, streamlining the development of the viral into a real-world clinical tool . Virus production for clinical gene therapy can be a limiting step because many virus generation protocols rely on labor-intensive, bench-scale methods; robust, cost-effective strategies for the delivery of clinical-grade viruses are thus essential for the future of gene therapy. A comprehensive picture of key aspects on the integration of upstream and downstream processing is addressed in this chapter, by describing the case study of recombinant budded baculoviruses for gene therapy; scalable methods are described in detail as well as mandatory characterization techniques for a proper and complete quality assessment of the viral vectors.
Original languageUnknown
Title of host publicationGene therapy of cancer: methods and protocols
EditorsW. Walther, U.S. Stein
Place of PublicationNew York
PublisherHumana Press
Pages447-470
ISBN (Print)978-1-934115-85-5/978-1-59745-561-9
Publication statusPublished - 1 Jan 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Number542
ISSN (Print)1064-3745

Cite this

Carrondo, M. J. T., & Alves, P. M. (2009). Virus Production for Clinical Gene Therapy. In W. Walther, & U. S. Stein (Eds.), Gene therapy of cancer: methods and protocols (pp. 447-470). (Methods in Molecular Biology; No. 542). New York: Humana Press.
Carrondo, Manuel José Teixeira ; Alves, Paula Maria. / Virus Production for Clinical Gene Therapy. Gene therapy of cancer: methods and protocols. editor / W. Walther ; U.S. Stein. New York : Humana Press, 2009. pp. 447-470 (Methods in Molecular Biology; 542).
@inbook{6c29985ef96c4de9a9a1543707bf7377,
title = "Virus Production for Clinical Gene Therapy",
abstract = "Gene therapy is becoming increasingly relevant for the treatment of prominent human diseases. Viral vectors are currently used in more than 50{\%} of the gene therapy clinical trials, most of them aimed at cancer diseases. Clearly, the increasing needs of high-quality viral preparations required the elimination of process bottlenecks, streamlining the development of the viral into a real-world clinical tool . Virus production for clinical gene therapy can be a limiting step because many virus generation protocols rely on labor-intensive, bench-scale methods; robust, cost-effective strategies for the delivery of clinical-grade viruses are thus essential for the future of gene therapy. A comprehensive picture of key aspects on the integration of upstream and downstream processing is addressed in this chapter, by describing the case study of recombinant budded baculoviruses for gene therapy; scalable methods are described in detail as well as mandatory characterization techniques for a proper and complete quality assessment of the viral vectors.",
author = "Carrondo, {Manuel Jos{\'e} Teixeira} and Alves, {Paula Maria}",
year = "2009",
month = "1",
day = "1",
language = "Unknown",
isbn = "978-1-934115-85-5/978-1-59745-561-9",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
number = "542",
pages = "447--470",
editor = "W. Walther and U.S. Stein",
booktitle = "Gene therapy of cancer: methods and protocols",

}

Carrondo, MJT & Alves, PM 2009, Virus Production for Clinical Gene Therapy. in W Walther & US Stein (eds), Gene therapy of cancer: methods and protocols. Methods in Molecular Biology, no. 542, Humana Press, New York, pp. 447-470.

Virus Production for Clinical Gene Therapy. / Carrondo, Manuel José Teixeira; Alves, Paula Maria.

Gene therapy of cancer: methods and protocols. ed. / W. Walther; U.S. Stein. New York : Humana Press, 2009. p. 447-470 (Methods in Molecular Biology; No. 542).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Virus Production for Clinical Gene Therapy

AU - Carrondo, Manuel José Teixeira

AU - Alves, Paula Maria

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Gene therapy is becoming increasingly relevant for the treatment of prominent human diseases. Viral vectors are currently used in more than 50% of the gene therapy clinical trials, most of them aimed at cancer diseases. Clearly, the increasing needs of high-quality viral preparations required the elimination of process bottlenecks, streamlining the development of the viral into a real-world clinical tool . Virus production for clinical gene therapy can be a limiting step because many virus generation protocols rely on labor-intensive, bench-scale methods; robust, cost-effective strategies for the delivery of clinical-grade viruses are thus essential for the future of gene therapy. A comprehensive picture of key aspects on the integration of upstream and downstream processing is addressed in this chapter, by describing the case study of recombinant budded baculoviruses for gene therapy; scalable methods are described in detail as well as mandatory characterization techniques for a proper and complete quality assessment of the viral vectors.

AB - Gene therapy is becoming increasingly relevant for the treatment of prominent human diseases. Viral vectors are currently used in more than 50% of the gene therapy clinical trials, most of them aimed at cancer diseases. Clearly, the increasing needs of high-quality viral preparations required the elimination of process bottlenecks, streamlining the development of the viral into a real-world clinical tool . Virus production for clinical gene therapy can be a limiting step because many virus generation protocols rely on labor-intensive, bench-scale methods; robust, cost-effective strategies for the delivery of clinical-grade viruses are thus essential for the future of gene therapy. A comprehensive picture of key aspects on the integration of upstream and downstream processing is addressed in this chapter, by describing the case study of recombinant budded baculoviruses for gene therapy; scalable methods are described in detail as well as mandatory characterization techniques for a proper and complete quality assessment of the viral vectors.

M3 - Chapter

SN - 978-1-934115-85-5/978-1-59745-561-9

T3 - Methods in Molecular Biology

SP - 447

EP - 470

BT - Gene therapy of cancer: methods and protocols

A2 - Walther, W.

A2 - Stein, U.S.

PB - Humana Press

CY - New York

ER -

Carrondo MJT, Alves PM. Virus Production for Clinical Gene Therapy. In Walther W, Stein US, editors, Gene therapy of cancer: methods and protocols. New York: Humana Press. 2009. p. 447-470. (Methods in Molecular Biology; 542).