@inbook{6c29985ef96c4de9a9a1543707bf7377,
title = "Virus production for clinical gene therapy",
abstract = "Gene therapy is becoming increasingly relevant for the treatment of prominent human diseases. Viral vectors are currently used in more than 50% of the gene therapy clinical trials, most of them aimed at cancer diseases. Clearly, the increasing needs of high-quality viral preparations required the elimination of process bottlenecks, streamlining the development of the viral into a real-world clinical tool . Virus production for clinical gene therapy can be a limiting step because many virus generation protocols rely on labor-intensive, bench-scale methods; robust, cost-effective strategies for the delivery of clinical-grade viruses are thus essential for the future of gene therapy. A comprehensive picture of key aspects on the integration of upstream and downstream processing is addressed in this chapter, by describing the case study of recombinant budded baculoviruses for gene therapy; scalable methods are described in detail as well as mandatory characterization techniques for a proper and complete quality assessment of the viral vectors.",
keywords = "Analytical tools, characterization, recombinant baculoviruses, upstream and downstream processing, viral vectors",
author = "Tiago Vicente and Cristina Peixoto and Carrondo, {Manuel J. T.} and Alves, {Paula M.}",
year = "2009",
doi = "10.1007/978-1-59745-561-9_24",
language = "English",
isbn = "978-1-934115-85-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
number = "542",
pages = "447--470",
editor = "Wolfgang Walther and Ulrike Stein",
booktitle = "Gene Therapy of Cancer",
address = "United States",
}
