Viability of the use of thin-film a-SiC: H photodiodes for protein identification

P. Louro, V. Silva, A. Karmali, M. Vieira

Research output: Contribution to journalArticle

Abstract

In this paper, we present a multilayer device based on a-Si:H/a-SiC:H that operates as photodetector and optical filter. The use of such device in protein detection applications is relevant in Fluorescence Resonance Energy Transfer (FRET) measurements. This method demands the detection of fluorescent signals located at specific wavelengths bands in the visible part of the electromagnetic spectrum. The device operates in the visible range with a selective sensitivity dependent on electrical and optical bias. Several nanosensors were tested with a commercial spectrophotometer to assess the performance of FRET signals using glucose solutions of different concentrations. The proposed device was used to demonstrate the possibility of FRET signals detection, using visible signals of similar wavelength and intensity. The device sensitivity was tuned to enhance the wavelength band of interest using steady state optical bias at 400 nm. Results show the ability of the device to detect signals in this range.

Original languageEnglish
Pages (from-to)228-233
Number of pages6
JournalPhysica Status Solidi (C) Current Topics In Solid State Physics
Volume11
Issue number2
DOIs
Publication statusPublished - 1 Feb 2014

Fingerprint

viability
photodiodes
proteins
resonance fluorescence
thin films
energy transfer
wavelengths
electromagnetic spectra
signal detection
optical filters
spectrophotometers
glucose
photometers
filters
sensitivity

Keywords

  • Glucose nanosensor
  • Optoelectronics

Cite this

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abstract = "In this paper, we present a multilayer device based on a-Si:H/a-SiC:H that operates as photodetector and optical filter. The use of such device in protein detection applications is relevant in Fluorescence Resonance Energy Transfer (FRET) measurements. This method demands the detection of fluorescent signals located at specific wavelengths bands in the visible part of the electromagnetic spectrum. The device operates in the visible range with a selective sensitivity dependent on electrical and optical bias. Several nanosensors were tested with a commercial spectrophotometer to assess the performance of FRET signals using glucose solutions of different concentrations. The proposed device was used to demonstrate the possibility of FRET signals detection, using visible signals of similar wavelength and intensity. The device sensitivity was tuned to enhance the wavelength band of interest using steady state optical bias at 400 nm. Results show the ability of the device to detect signals in this range.",
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Viability of the use of thin-film a-SiC : H photodiodes for protein identification. / Louro, P.; Silva, V.; Karmali, A.; Vieira, M.

In: Physica Status Solidi (C) Current Topics In Solid State Physics, Vol. 11, No. 2, 01.02.2014, p. 228-233.

Research output: Contribution to journalArticle

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