Vernonia britteniana Root Phytochemical Studies, In Vitro Cercaricidal Activity on the Larval Stage of Schistosoma mansoni and Antioxidant Activities

Maria dos Anjos Valente, Pedro Ferreira, Katelene Lima, Isabel B. Moreira da Silva, Paula Nobre, Isabel Neto, Mavilde Pires, Berta São Braz, Rita Serrano, Silvana Belo, Olga Silva

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Abstract

Vernonia britteniana Hiern. (Asteraceae) is a medicinal plant used in traditional Angolan medicine against schistosomiasis. Our study aimed to investigate the phytochemical composition and the cercaricidal and antioxidant activities in vitro of a traditional herbal preparation (Water-Vbr) and a 70% hydroethanolic extract (EtOH70%-Vbr) prepared with this medicinal plant. The activity of the extracts against Schistosoma mansoni cercariae was assessed at different extract concentrations (500, 438, and 125 µg/mL) and at different time intervals, and the phytochemical profiles were obtained by LC-UV-ESI/MS-MS. In addition, the major chemical classes of the identified metabolites were quantified by colorimetry, and the antioxidant potential was assessed using the DPPH and FRAP methods. After 30 min, 100% cercarial mortality was observed at a concentration of 500 μg/mL after exposure, and after 120 min, an LC50 of 438 μg/mL was observed for both extracts. Phenolic acid derivatives (chlorogenic acid, caffeic acid; 3,4-di-O-caffeoylquinic acid; 3,5-di-O-caffeoylquinic acid; and 4,5-di-O-caffeoylquinic acid) and triterpenoids (stigmastane-type steroidal saponins; vernoamyoside D and vernonioside D1; vernoamyoside B; and vernoniamyoside A and C) were identified as the main secondary metabolites. The Water-Vbr extract showed the highest antioxidant activity—DPPH: IC50 = 1.769 ± 0.049 µg/mL; FRAP: mean = 320.80 ± 5.1325 µgAAE/g.

Original languageEnglish
Article number1788
Number of pages14
JournalPlants
Volume12
Issue number9
DOIs
Publication statusPublished - May 2023

Keywords

  • Angola
  • antioxidant activity
  • cercaricidal activity
  • medicinal plant
  • Schistosoma mansoni
  • traditional medicine
  • vernoamyoside D
  • Vernonia britteniana

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