Variable surface glycoprotein from trypanosoma brucei undergoes cleavage by matrix metalloproteinases: an in silico approach

Claudia Jassica Gonçalves Moreno, Torres, T. M., MS Silva

Research output: Contribution to journalArticle

Abstract

In order to survive as extracellular parasites in the mammalian host environment, Trypanosoma brucei has developed efficient mechanisms of immune system evasion, which include the abundant expression of a variable surface glycoprotein (VSG) coat. VSGs are anchored in the parasite membrane by covalent C-terminal binding to glycosylphosphatidylinositol and may be periodically removed by a phospholipase C (PLC) and a major surface protein (TbMSP). VSG molecules show extraordinary antigenic diversity and a comparative analysis of protein sequences suggests that conserved elements may be a suitable target against African trypanosomiasis. However, the cleavage mechanisms of these molecules remain unclear. Moreover, in protozoan infections, including those caused by Trypanosoma brucei, it is possible to observe an increased expression of the matrix metalloproteinases (MMPs). To address the cleavage mechanism of VSGs, the PROSPER server was used for the identification of VSG sequence cleavage sites. After data compilation, it was
observed that 64 VSG consensus sequences showed a high conservation of hydrophobic residues, such as valine (V), methionine (M), leucine (L) and isoleucine (I) in the fifth position—the exact location of the cleavage site. In addition, the PROSPER server identified conserved cleavage site portions of
VSG proteins recognized by three matrix metalloproteases (gelatinases: MMP-2, MMP-3 and MMP-9). However, further biological studies are needed in order to analyze and confirm this prediction.
Original languageEnglish
Article number178
Pages (from-to)178-187
Number of pages9
JournalPathogens
VolumeVol. 8
Issue numbern.º 4
DOIs
Publication statusPublished - 8 Oct 2019

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Keywords

  • African trypanosomiasis
  • Trypanosoma brucei
  • Major surface protein
  • Matrix metalloproteinases
  • Phospholipase C
  • Variable surface glycoproteins

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