Vanadium Complexes as Prospective Therapeutics: Structural Characterization of a V-IV Lysozyme Adduct

Marino F. A. Santos, Isabel Lopes Correia, Ana Isabel Nobre Martins Aguiar de Oliveira Ricardo, Eugenio Garribba, Joao Costa Pessoa, Teresa Santos-Silva

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The biological activity of vanadium complexes, namely, as insulin enhancers, is well known. We report a combined X-ray crystallography, electron paramagnetic resonance, and density functional theory study of the interaction of vanadium picolinate complexes with hen egg white lysozyme (HEWL). We show that the (VO)-O-IV(pic)(2) complex covalently binds to the COO- group of the side chain of Asp52 of HEWL. The long V-IV=O bond obtained in the X-ray study is explained to be due to reduction of V-IV to V-III during exposure of the crystals to the intense X-ray beam.

Original languageEnglish
Pages (from-to)3293-3297
Number of pages5
JournalEuropean Journal of Inorganic Chemistry
Issue number21
DOIs
Publication statusPublished - Jul 2014

Keywords

  • Vanadium
  • Protein adducts
  • Medicinal chemistry
  • EPR spectroscopy
  • Density functional calculations
  • HYPERFINE COUPLING-CONSTANTS
  • BLOOD-SERUM
  • (VO)-O-IV COMPLEXES
  • CRYSTAL-STRUCTURE
  • AQUEOUS-SOLUTION
  • AMINO-ACIDS
  • SOLID-STATE
  • TRANSFERRIN
  • OXOVANADIUM(IV)
  • PREDICTION

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