Valorization of platelets with no therapeutic value with Pulsed Electric Fields

D. Rego, L. M. Redondo, A. P. Sousa, S. Abreu, M. Serra, V. E. Santo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Platelets are blood components with high biomedical potential due to their physiological role in wound healing and their rich growth factor content. This paper describes proof of concept experiments aimed to produce a new blood derived product by applying Pulsed Electric Fields (PEF) in platelet concentrates with no therapeutic value for transfusion medicine. A human platelet concentrate suspension was subjected to a PEF treatment of 1 pulse, 5 kV/cm, with 2 μs. Release of Platelet Derived Growth Factor (PDGF) from the electroporated platelets was measured by ELISA. Furthermore, the biological activity of the obtained blood derived product was characterized. Human mesenchymal stem cells (hMSC) were cultured in the presence of the proteins released from the platelets after PEF application and evaluated for their expansion potential. Results show that platelets concentrates submitted to a single PEF treatment can release PDGF to the supernatant. Protein release from the single and transient PEF cycle was confirmed by the expansion of hMSC cultured with a medium supplemented with platelet releasate obtained from electroporated platelets. These results support the valorization of platelet concentrates with no transfusion value that would otherwise be discarded. Applying PEF might allow the development of alternative releasate for different biomedical applications, namely stem cells culture.

Original languageEnglish
Title of host publication6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538685068
DOIs
Publication statusPublished - 15 Apr 2019
Event6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Lisbon, Portugal
Duration: 22 Feb 201923 Feb 2019

Publication series

Name6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings

Conference

Conference6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019
CountryPortugal
CityLisbon
Period22/02/1923/02/19

Fingerprint Dive into the research topics of 'Valorization of platelets with no therapeutic value with Pulsed Electric Fields'. Together they form a unique fingerprint.

  • Cite this

    Rego, D., Redondo, L. M., Sousa, A. P., Abreu, S., Serra, M., & Santo, V. E. (2019). Valorization of platelets with no therapeutic value with Pulsed Electric Fields. In 6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings [8692500] (6th IEEE Portuguese Meeting on Bioengineering, ENBENG 2019 - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ENBENG.2019.8692500