Valorization of fatty acids-containing wastes and byproducts into short- and medium-chain length polyhydroxyalkanoates

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24 Citations (Scopus)

Abstract

Olive oil distillate (OOD), biodiesel fatty acids-byproduct (FAB) and used cooking oil (UCO) were tested as inexpensive carbon sources for the production of polyhydroxyalkanoates (PHA) with different composition using twelve bacterial strains. OOD and FAB were exploited for the first time as alternative substrates for PHA production. UCO, OOD and FAB were used by Cupriavidus necator and Pseudomonas oleovorans to synthesize the homopolymer poly-3-hydroxybutyrate, while Pseudomonas resinovorans and Pseudomonas citronellolis produced mcl-PHA polymers mainly composed of hydroxyoctanoate and hydroxydecanoate monomers. The highest polymer content in the biomass was obtained for C. necator (62. wt.%) cultivated on OOD. Relatively high mcl-PHA content (28-31. wt.%) was reached by P. resinovorans cultivated in OOD. This study shows, for the first time, that OOD is a promising substrate for PHA production since it gives high polymer yields and allows for the synthesis of different polymers (scl- or mcl-PHA) by selection of the adequate strains.

Original languageEnglish
Pages (from-to)206-215
Number of pages10
JournalNew Biotechnology
Volume33
Issue number1
DOIs
Publication statusPublished - 25 Jan 2016

Fingerprint

Polyhydroxyalkanoates
Olive oil
Chain length
Fatty acids
Byproducts
Fatty Acids
Polymers
Cupriavidus necator
Cooking
Pseudomonas
Pseudomonas oleovorans
Oils
Biofuels
Substrates
Biodiesel
Homopolymerization
Biomass
Olive Oil
Carbon
Monomers

Keywords

  • SPENT COFFEE GROUNDS
  • PSEUDOMONAS-OLEOVORANS
  • POLY(HYDROXYALKANOATE) BIOSYNTHESIS
  • DEODORIZER DISTILLATES
  • OIL
  • POLYESTERS

Cite this

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title = "Valorization of fatty acids-containing wastes and byproducts into short- and medium-chain length polyhydroxyalkanoates",
abstract = "Olive oil distillate (OOD), biodiesel fatty acids-byproduct (FAB) and used cooking oil (UCO) were tested as inexpensive carbon sources for the production of polyhydroxyalkanoates (PHA) with different composition using twelve bacterial strains. OOD and FAB were exploited for the first time as alternative substrates for PHA production. UCO, OOD and FAB were used by Cupriavidus necator and Pseudomonas oleovorans to synthesize the homopolymer poly-3-hydroxybutyrate, while Pseudomonas resinovorans and Pseudomonas citronellolis produced mcl-PHA polymers mainly composed of hydroxyoctanoate and hydroxydecanoate monomers. The highest polymer content in the biomass was obtained for C. necator (62. wt.{\%}) cultivated on OOD. Relatively high mcl-PHA content (28-31. wt.{\%}) was reached by P. resinovorans cultivated in OOD. This study shows, for the first time, that OOD is a promising substrate for PHA production since it gives high polymer yields and allows for the synthesis of different polymers (scl- or mcl-PHA) by selection of the adequate strains.",
keywords = "SPENT COFFEE GROUNDS, PSEUDOMONAS-OLEOVORANS, POLY(HYDROXYALKANOATE) BIOSYNTHESIS, DEODORIZER DISTILLATES, OIL, POLYESTERS",
author = "Cruz, {Madalena V.} and Filomena Freitas and Alexandre Paiva and Francisca Mano and Madalena Dion{\'i}sio and Ramos, {Ana Maria} and Reis, {Maria D'ascens{\~a}o Carvalho Fernandes Miranda}",
note = "Fundacao para a Ciencia e a Tecnologia - SFRH/BPD/72280/2010 ; SFRH/BPD/44946/2008 ; SFRH/BD/72142/2010 QREN (PEst-C/EQB/LA0006/2013)",
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doi = "10.1016/j.nbt.2015.05.005",
language = "English",
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journal = "New Biotechnology",
issn = "1871-6784",
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TY - JOUR

T1 - Valorization of fatty acids-containing wastes and byproducts into short- and medium-chain length polyhydroxyalkanoates

AU - Cruz, Madalena V.

AU - Freitas, Filomena

AU - Paiva, Alexandre

AU - Mano, Francisca

AU - Dionísio, Madalena

AU - Ramos, Ana Maria

AU - Reis, Maria D'ascensão Carvalho Fernandes Miranda

N1 - Fundacao para a Ciencia e a Tecnologia - SFRH/BPD/72280/2010 ; SFRH/BPD/44946/2008 ; SFRH/BD/72142/2010 QREN (PEst-C/EQB/LA0006/2013)

PY - 2016/1/25

Y1 - 2016/1/25

N2 - Olive oil distillate (OOD), biodiesel fatty acids-byproduct (FAB) and used cooking oil (UCO) were tested as inexpensive carbon sources for the production of polyhydroxyalkanoates (PHA) with different composition using twelve bacterial strains. OOD and FAB were exploited for the first time as alternative substrates for PHA production. UCO, OOD and FAB were used by Cupriavidus necator and Pseudomonas oleovorans to synthesize the homopolymer poly-3-hydroxybutyrate, while Pseudomonas resinovorans and Pseudomonas citronellolis produced mcl-PHA polymers mainly composed of hydroxyoctanoate and hydroxydecanoate monomers. The highest polymer content in the biomass was obtained for C. necator (62. wt.%) cultivated on OOD. Relatively high mcl-PHA content (28-31. wt.%) was reached by P. resinovorans cultivated in OOD. This study shows, for the first time, that OOD is a promising substrate for PHA production since it gives high polymer yields and allows for the synthesis of different polymers (scl- or mcl-PHA) by selection of the adequate strains.

AB - Olive oil distillate (OOD), biodiesel fatty acids-byproduct (FAB) and used cooking oil (UCO) were tested as inexpensive carbon sources for the production of polyhydroxyalkanoates (PHA) with different composition using twelve bacterial strains. OOD and FAB were exploited for the first time as alternative substrates for PHA production. UCO, OOD and FAB were used by Cupriavidus necator and Pseudomonas oleovorans to synthesize the homopolymer poly-3-hydroxybutyrate, while Pseudomonas resinovorans and Pseudomonas citronellolis produced mcl-PHA polymers mainly composed of hydroxyoctanoate and hydroxydecanoate monomers. The highest polymer content in the biomass was obtained for C. necator (62. wt.%) cultivated on OOD. Relatively high mcl-PHA content (28-31. wt.%) was reached by P. resinovorans cultivated in OOD. This study shows, for the first time, that OOD is a promising substrate for PHA production since it gives high polymer yields and allows for the synthesis of different polymers (scl- or mcl-PHA) by selection of the adequate strains.

KW - SPENT COFFEE GROUNDS

KW - PSEUDOMONAS-OLEOVORANS

KW - POLY(HYDROXYALKANOATE) BIOSYNTHESIS

KW - DEODORIZER DISTILLATES

KW - OIL

KW - POLYESTERS

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U2 - 10.1016/j.nbt.2015.05.005

DO - 10.1016/j.nbt.2015.05.005

M3 - Article

VL - 33

SP - 206

EP - 215

JO - New Biotechnology

JF - New Biotechnology

SN - 1871-6784

IS - 1

ER -