TY - JOUR
T1 - Valorisation of grape pomace: Fractionation of bioactive flavan-3-ols by membrane processing
AU - Syed, Usman Taqui
AU - Brazinha, Carla
AU - Crespo, João G.
AU - Ricardo-da-Silva, Jorge M.
N1 - The authors would like to acknowledge Portuguese Foundation for Science and Technology FCT for the Pos Doctoral Fellow grant SFRH/BPD/79533/2011 of Carla Brazinha. Executive Agency for Education, Audiovisual & Culture (EACEA) of the European Commission is also gratefully acknowledged for the Erasmus Mundus scholarship to Syed Usman Taqui under the Erasmus Mundus Master in Membrane Engineering program. The authors would also like to acknowledge Eng Luis Patrao from Esporao, S.A. for his essential support for carrying out this work, including supplying grape pomace. The assistance provided by the lab technicians Mafalda Cadima at LAQV-Requimte, FCT-Universidade Nova de Lisboa and also Diana Faria and Daniel Duarte at Institute Superior de Agronomia, Universidade de Lisboa is gratefully acknowledged.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - This work discusses the development of a process which aims at the production of high-value extracts, a food nutraceutical ingredient, rich in bioactive monomeric flavan-3-ols, by using low-value grape pomace as a raw material. As a first step, an extraction process using biocompatible solvents was optimised. The extract obtained was then fractionated using membrane technologies that allow obtaining target fractions of bioactive compounds and also to the recycle of the solvent. A detailed chemical characterisation of these fractions was also accomplished. The fractionation process achieved by dia-nanofiltration enabled to reject 40% of the monomeric flavan-3-ols in the permeate, with a low use of solvent (diafiltration volume of 2). The fractionated permeate was further concentrated by reverse osmosis, allowing for 90% re-use of the solvent. Considering this excellent result, higher dia-nanofiltration volumes may be used without additional solvent expenditure. Considering these results, a diafiltration volume of 5 may be used, leading to an estimated overall process rejection of 15% of monomeric flavan-3-ols.
AB - This work discusses the development of a process which aims at the production of high-value extracts, a food nutraceutical ingredient, rich in bioactive monomeric flavan-3-ols, by using low-value grape pomace as a raw material. As a first step, an extraction process using biocompatible solvents was optimised. The extract obtained was then fractionated using membrane technologies that allow obtaining target fractions of bioactive compounds and also to the recycle of the solvent. A detailed chemical characterisation of these fractions was also accomplished. The fractionation process achieved by dia-nanofiltration enabled to reject 40% of the monomeric flavan-3-ols in the permeate, with a low use of solvent (diafiltration volume of 2). The fractionated permeate was further concentrated by reverse osmosis, allowing for 90% re-use of the solvent. Considering this excellent result, higher dia-nanofiltration volumes may be used without additional solvent expenditure. Considering these results, a diafiltration volume of 5 may be used, leading to an estimated overall process rejection of 15% of monomeric flavan-3-ols.
KW - Biorefinery
KW - Diafiltration
KW - Grape pomace
KW - Membrane processing
KW - Phenolic compounds
UR - http://www.scopus.com/inward/record.url?scp=84989939517&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2016.07.039
DO - 10.1016/j.seppur.2016.07.039
M3 - Article
AN - SCOPUS:84989939517
SN - 1383-5866
VL - 172
SP - 404
EP - 414
JO - Separation and Purification Technology
JF - Separation and Purification Technology
ER -