Validating silicon polytrodes with paired juxtacellular recordings: Method and dataset

Joana P. Neto, Gonçalo Lopes, João Frazão, Joana Nogueira, Pedro Lacerda, Pedro Baião, Arno Aarts, Alexandru Andrei, Silke Musa, Elvira Fortunato, Pedro Barquinha, Adam R. Kampff

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Cross-validating new methods for recording neural activity is necessary to accurately interpret and compare the signals they measure. Here we describe a procedure for precisely aligning two probes for in vivo "paired-recordings" such that the spiking activity of a single neuron is monitored with both a dense extracellular silicon polytrode and a juxtacellular micropipette. Our new method allows for efficient, reliable, and automated guidance of both probes to the same neural structure with micrometer resolution. We also describe a new dataset of paired-recordings, which is available online. We propose that our novel targeting system, and ever expanding cross-validation dataset, will be vital to the development of new algorithms for automatically detecting/sorting single-units, characterizing new electrode materials/ designs, and resolving nagging questions regarding the origin and nature of extracellular neural signals.

Original languageEnglish
Pages (from-to)892-903
Number of pages12
JournalJournal of Neurophysiology
Volume116
Issue number2
DOIs
Publication statusPublished - 1 Aug 2016

Keywords

  • Extracellular action potential
  • Ground truth
  • Juxtacellular recording
  • Polytrodes
  • Spike sorting

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