Use of gold nanoparticles as additives in protein crystallization

Diana Ribeiro, Alina Kulakova, Pedro Quaresma, Eulália Pereira, Cecília Bonifácio, Maria João Romão, Ricardo Franco, Ana Luísa Carvalho

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Gold nanoparticles (AuNPs) exhibit unique properties that have made them a very attractive material for application in biological assays. Given the potentially interesting interactions between AuNPs and biological macromolecules, we investigated AuNPs-induced protein crystal growth. Differently functionalized AuNPs were tested as additives in cocrystallization studies with model proteins (hen egg white lysozyme (HEWL), ribonuclease A (RNase A), and proteinase K) as well as with case studies where there were problems in obtaining well-diffracting crystals. Trials were performed considering different crystallization drawbacks, from total absence of crystals to improvement of crystal morphology, size, twinning, and number of crystals per drop. Improvement of some of these factors was observed in the cases of HEWL, RNase A, phenylalanine hydroxylase (PAH), myoglobin, native aldehyde oxidase (AOH), and human albumin. In these proteins, the presence of the AuNPs promoted an increase in the size and/or better crystal morphology. From the systematic trials and subsequent observations, it can be concluded that the introduction of AuNPs should definitely be considered in crystal optimization trials to improve previously determined crystallization conditions.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalCrystal Growth & Design
Volume14
Issue number1
DOIs
Publication statusPublished - 2 Jan 2014

Fingerprint

Crystallization
Gold
crystallization
gold
Nanoparticles
proteins
Proteins
nanoparticles
Crystals
crystal morphology
eggs
lysozyme
crystals
Pancreatic Ribonuclease
protein crystal growth
Muramidase
myoglobin
phenylalanine
oxidase
twinning

Keywords

  • Biological macromolecule
  • Crystal morphologies
  • Crystal optimization
  • Crystallization conditions
  • Hen egg white lysozyme
  • Phenylalanine hydroxylase
  • Protein crystal growth
  • Protein crystallization

Cite this

Ribeiro, D., Kulakova, A., Quaresma, P., Pereira, E., Bonifácio, C., Romão, M. J., ... Carvalho, A. L. (2014). Use of gold nanoparticles as additives in protein crystallization. Crystal Growth & Design, 14(1), 222-227. https://doi.org/10.1021/cg4014398
Ribeiro, Diana ; Kulakova, Alina ; Quaresma, Pedro ; Pereira, Eulália ; Bonifácio, Cecília ; Romão, Maria João ; Franco, Ricardo ; Carvalho, Ana Luísa. / Use of gold nanoparticles as additives in protein crystallization. In: Crystal Growth & Design. 2014 ; Vol. 14, No. 1. pp. 222-227.
@article{a23b71772921466a9ae31b29fa94b626,
title = "Use of gold nanoparticles as additives in protein crystallization",
abstract = "Gold nanoparticles (AuNPs) exhibit unique properties that have made them a very attractive material for application in biological assays. Given the potentially interesting interactions between AuNPs and biological macromolecules, we investigated AuNPs-induced protein crystal growth. Differently functionalized AuNPs were tested as additives in cocrystallization studies with model proteins (hen egg white lysozyme (HEWL), ribonuclease A (RNase A), and proteinase K) as well as with case studies where there were problems in obtaining well-diffracting crystals. Trials were performed considering different crystallization drawbacks, from total absence of crystals to improvement of crystal morphology, size, twinning, and number of crystals per drop. Improvement of some of these factors was observed in the cases of HEWL, RNase A, phenylalanine hydroxylase (PAH), myoglobin, native aldehyde oxidase (AOH), and human albumin. In these proteins, the presence of the AuNPs promoted an increase in the size and/or better crystal morphology. From the systematic trials and subsequent observations, it can be concluded that the introduction of AuNPs should definitely be considered in crystal optimization trials to improve previously determined crystallization conditions.",
keywords = "Biological macromolecule , Crystal morphologies , Crystal optimization , Crystallization conditions , Hen egg white lysozyme , Phenylalanine hydroxylase , Protein crystal growth , Protein crystallization",
author = "Diana Ribeiro and Alina Kulakova and Pedro Quaresma and Eul{\'a}lia Pereira and Cec{\'i}lia Bonif{\'a}cio and Rom{\~a}o, {Maria Jo{\~a}o} and Ricardo Franco and Carvalho, {Ana Lu{\'i}sa}",
note = "WOS:000329337000029",
year = "2014",
month = "1",
day = "2",
doi = "10.1021/cg4014398",
language = "English",
volume = "14",
pages = "222--227",
journal = "Crystal Growth & Design",
issn = "1528-7483",
publisher = "The American Chemical Society",
number = "1",

}

Ribeiro, D, Kulakova, A, Quaresma, P, Pereira, E, Bonifácio, C, Romão, MJ, Franco, R & Carvalho, AL 2014, 'Use of gold nanoparticles as additives in protein crystallization', Crystal Growth & Design, vol. 14, no. 1, pp. 222-227. https://doi.org/10.1021/cg4014398

Use of gold nanoparticles as additives in protein crystallization. / Ribeiro, Diana; Kulakova, Alina; Quaresma, Pedro; Pereira, Eulália; Bonifácio, Cecília; Romão, Maria João; Franco, Ricardo; Carvalho, Ana Luísa.

In: Crystal Growth & Design, Vol. 14, No. 1, 02.01.2014, p. 222-227.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of gold nanoparticles as additives in protein crystallization

AU - Ribeiro, Diana

AU - Kulakova, Alina

AU - Quaresma, Pedro

AU - Pereira, Eulália

AU - Bonifácio, Cecília

AU - Romão, Maria João

AU - Franco, Ricardo

AU - Carvalho, Ana Luísa

N1 - WOS:000329337000029

PY - 2014/1/2

Y1 - 2014/1/2

N2 - Gold nanoparticles (AuNPs) exhibit unique properties that have made them a very attractive material for application in biological assays. Given the potentially interesting interactions between AuNPs and biological macromolecules, we investigated AuNPs-induced protein crystal growth. Differently functionalized AuNPs were tested as additives in cocrystallization studies with model proteins (hen egg white lysozyme (HEWL), ribonuclease A (RNase A), and proteinase K) as well as with case studies where there were problems in obtaining well-diffracting crystals. Trials were performed considering different crystallization drawbacks, from total absence of crystals to improvement of crystal morphology, size, twinning, and number of crystals per drop. Improvement of some of these factors was observed in the cases of HEWL, RNase A, phenylalanine hydroxylase (PAH), myoglobin, native aldehyde oxidase (AOH), and human albumin. In these proteins, the presence of the AuNPs promoted an increase in the size and/or better crystal morphology. From the systematic trials and subsequent observations, it can be concluded that the introduction of AuNPs should definitely be considered in crystal optimization trials to improve previously determined crystallization conditions.

AB - Gold nanoparticles (AuNPs) exhibit unique properties that have made them a very attractive material for application in biological assays. Given the potentially interesting interactions between AuNPs and biological macromolecules, we investigated AuNPs-induced protein crystal growth. Differently functionalized AuNPs were tested as additives in cocrystallization studies with model proteins (hen egg white lysozyme (HEWL), ribonuclease A (RNase A), and proteinase K) as well as with case studies where there were problems in obtaining well-diffracting crystals. Trials were performed considering different crystallization drawbacks, from total absence of crystals to improvement of crystal morphology, size, twinning, and number of crystals per drop. Improvement of some of these factors was observed in the cases of HEWL, RNase A, phenylalanine hydroxylase (PAH), myoglobin, native aldehyde oxidase (AOH), and human albumin. In these proteins, the presence of the AuNPs promoted an increase in the size and/or better crystal morphology. From the systematic trials and subsequent observations, it can be concluded that the introduction of AuNPs should definitely be considered in crystal optimization trials to improve previously determined crystallization conditions.

KW - Biological macromolecule

KW - Crystal morphologies

KW - Crystal optimization

KW - Crystallization conditions

KW - Hen egg white lysozyme

KW - Phenylalanine hydroxylase

KW - Protein crystal growth

KW - Protein crystallization

UR - http://www.scopus.com/inward/record.url?scp=84891715021&partnerID=8YFLogxK

U2 - 10.1021/cg4014398

DO - 10.1021/cg4014398

M3 - Article

VL - 14

SP - 222

EP - 227

JO - Crystal Growth & Design

JF - Crystal Growth & Design

SN - 1528-7483

IS - 1

ER -

Ribeiro D, Kulakova A, Quaresma P, Pereira E, Bonifácio C, Romão MJ et al. Use of gold nanoparticles as additives in protein crystallization. Crystal Growth & Design. 2014 Jan 2;14(1):222-227. https://doi.org/10.1021/cg4014398