Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino; Helena Barroso; Maria De Fátima Gonçalves; Sofia Marques Silva; Carlos Novo; Perpétua Gomes; Ricardo Jorge Camacho; Nuno Taveira

doi:10.1128/JCM.44.2.607-611.2006

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino, Helena Barroso, Maria De Fátima Gonçalves, Sofia Marques Silva, Carlos Novo, Perpétua Gomes, Ricardo Jorge Camacho, Nuno Taveira

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

Original language	English
Pages (from-to)	607-611
Number of pages	5
Journal	Journal Of Clinical Microbiology
Volume	Vol. 44
Issue number	n.º 2
DOIs	https://doi.org/10.1128/JCM.44.2.607-611.2006
Publication status	Published - Feb 2006

Keywords

Enzyme-Linked Immunosorbent Assay
Gene Products, env
HIV Antibodies
HIV Antigens
HIV Infections
HIV-2
Humans
Protein Precursors
Recombinant Proteins
Sensitivity and Specificity
env Gene Products, Human Immunodeficiency Virus
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JCM.44.2.607-611.2006

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Marcelino, J. M., Barroso, H., Gonçalves, M. D. F., Silva, S. M., Novo, C., Gomes, P., Camacho, R. J., & Taveira, N. (2006). Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins. Journal Of Clinical Microbiology, Vol. 44(n.º 2), 607-611. https://doi.org/10.1128/JCM.44.2.607-611.2006

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abstract = "A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.",

keywords = "Enzyme-Linked Immunosorbent Assay, Gene Products, env, HIV Antibodies, HIV Antigens, HIV Infections, HIV-2, Humans, Protein Precursors, Recombinant Proteins, Sensitivity and Specificity, env Gene Products, Human Immunodeficiency Virus, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't",

author = "Marcelino, {Jos{\'e} Maria} and Helena Barroso and Gon{\c c}alves, {Maria De F{\'a}tima} and Silva, {Sofia Marques} and Carlos Novo and Perp{\'e}tua Gomes and Camacho, {Ricardo Jorge} and Nuno Taveira",

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Marcelino, JM, Barroso, H, Gonçalves, MDF, Silva, SM, Novo, C , Gomes, P, Camacho, RJ & Taveira, N 2006, 'Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins', Journal Of Clinical Microbiology, vol. Vol. 44, no. n.º 2, pp. 607-611. https://doi.org/10.1128/JCM.44.2.607-611.2006

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins. / Marcelino, José Maria; Barroso, Helena; Gonçalves, Maria De Fátima et al.
In: Journal Of Clinical Microbiology, Vol. Vol. 44, No. n.º 2, 02.2006, p. 607-611.

Research output: Contribution to journal › Article › peer-review

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AU - Marcelino, José Maria

AU - Barroso, Helena

AU - Gonçalves, Maria De Fátima

AU - Silva, Sofia Marques

AU - Novo, Carlos

AU - Gomes, Perpétua

AU - Camacho, Ricardo Jorge

AU - Taveira, Nuno

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N2 - A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

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KW - HIV Antigens

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KW - Humans

KW - Protein Precursors

KW - Recombinant Proteins

KW - Sensitivity and Specificity

KW - env Gene Products, Human Immunodeficiency Virus

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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Marcelino JM, Barroso H, Gonçalves MDF, Silva SM, Novo C , Gomes P et al. Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins. Journal Of Clinical Microbiology. 2006 Feb;Vol. 44(n.º 2):607-611. doi: 10.1128/JCM.44.2.607-611.2006

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

Abstract

Keywords

UN SDGs

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