Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino, Helena Barroso, Maria De Fátima Gonçalves, Sofia Marques Silva, Carlos Novo, Perpétua Gomes, Ricardo Jorge Camacho, Nuno Taveira

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

Original languageEnglish
Pages (from-to)607-611
Number of pages5
JournalJournal Of Clinical Microbiology
VolumeVol. 44
Issue numbern.º 2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • Enzyme-Linked Immunosorbent Assay
  • Gene Products, env
  • HIV Antibodies
  • HIV Antigens
  • HIV Infections
  • HIV-2
  • Humans
  • Protein Precursors
  • Recombinant Proteins
  • Sensitivity and Specificity
  • env Gene Products, Human Immunodeficiency Virus
  • Evaluation Studies
  • Journal Article
  • Research Support, Non-U.S. Gov't

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