Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Catarina Milheiriço; Duarte Emanuel Soeiro de Carvalho Oliveira; Herminia Garcez Lencastre

doi:10.1128/AAC.00275-07

Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Catarina Milheiriço, Duarte Emanuel Soeiro de Carvalho Oliveira, Herminia Garcez Lencastre

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

411 Citations (Scopus)

Abstract

Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

Original language	English
Pages (from-to)	3374-3377
Journal	Antimicrobial Agents and Chemotherapy
Volume	51
Issue number	9
DOIs	https://doi.org/10.1128/AAC.00275-07
Publication status	Published - 1 Sep 2007

Access to Document

10.1128/AAC.00275-07

Cite this

@article{987839c017db4c82b00bb39fa684f46b,

title = "Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus",

abstract = "Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.",

keywords = "COMMUNITY, MRSA, VARIANTS, CLONES, GENOME, CASSETTE CHROMOSOME MEC, RESISTANCE",

author = "Catarina Milheiri{\c c}o and Oliveira, {Duarte Emanuel Soeiro de Carvalho} and Lencastre, {Herminia Garcez}",

note = "Milheirico, C.; Oliveira, D. C. - autores ITQB",

year = "2007",

month = sep,

day = "1",

doi = "10.1128/AAC.00275-07",

language = "English",

volume = "51",

pages = "3374--3377",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "9",

}

TY - JOUR

T1 - Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

AU - Milheiriço, Catarina

AU - Oliveira, Duarte Emanuel Soeiro de Carvalho

AU - Lencastre, Herminia Garcez

N1 - Milheirico, C.; Oliveira, D. C. - autores ITQB

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

AB - Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

KW - COMMUNITY

KW - MRSA

KW - VARIANTS

KW - CLONES

KW - GENOME

KW - CASSETTE CHROMOSOME MEC

KW - RESISTANCE

U2 - 10.1128/AAC.00275-07

DO - 10.1128/AAC.00275-07

M3 - Article

VL - 51

SP - 3374

EP - 3377

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 9

ER -

Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Abstract

Access to Document

Fingerprint

Cite this