Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Catarina Milheiriço; Duarte Emanuel Soeiro de Carvalho Oliveira; Herminia Garcez Lencastre

doi:10.1128/AAC.00275-07

Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Catarina Milheiriço, Duarte Emanuel Soeiro de Carvalho Oliveira, Herminia Garcez Lencastre

Instituto de Tecnologia Química e Biológica António Xavier (ITQB)

Research output: Contribution to journal › Article › peer-review

448 Citations (Scopus)

Abstract

Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

Original language	English
Pages (from-to)	3374-3377
Journal	Antimicrobial Agents and Chemotherapy
Volume	51
Issue number	9
DOIs	https://doi.org/10.1128/AAC.00275-07
Publication status	Published - 1 Sept 2007

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title = "Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus",

abstract = "Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.",

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AB - Staphylococcal cassette chromosome mec (SCCmec) typing is important for the identification and definition of methicillin-resistant Staphylococcus aureus clones, and for routine purposes, multiplex PCR assays are the most adequate for SCCmec typing. Here, we describe an update to the multiplex PCR strategy for SCCmec typing that we described in 2002 so that SCCmec types IV and V may be properly identified.

KW - COMMUNITY

KW - MRSA

KW - VARIANTS

KW - CLONES

KW - GENOME

KW - CASSETTE CHROMOSOME MEC

KW - RESISTANCE

U2 - 10.1128/AAC.00275-07

DO - 10.1128/AAC.00275-07

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

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ER -

Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus

Abstract

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