Unveiling PHA-storing populations using molecular methods

Diogo Queirós, Paulo Alexandre da Costa Lemos, Simona Rossetti, Luísa S. Serafim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process.
Original languageEnglish
Pages (from-to)10433-10446
JournalApplied Microbiology and Biotechnology
Volume99
Issue number24
DOIs
Publication statusPublished - 2015

Fingerprint

Polyhydroxyalkanoates
Population
Polymerase Chain Reaction
Denaturing Gradient Gel Electrophoresis
Fluorescence In Situ Hybridization
Restriction Fragment Length Polymorphisms

Keywords

  • Mixed microbial culture
  • Polyhydroxyalkanoates
  • Ecology
  • Hybridization
  • PCR-based methods

Cite this

Queirós, Diogo ; Lemos, Paulo Alexandre da Costa ; Rossetti, Simona ; Serafim, Luísa S. / Unveiling PHA-storing populations using molecular methods. In: Applied Microbiology and Biotechnology. 2015 ; Vol. 99, No. 24. pp. 10433-10446.
@article{6d6787db679d46aebea2cd5233fcaade,
title = "Unveiling PHA-storing populations using molecular methods",
abstract = "Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process.",
keywords = "Mixed microbial culture , Polyhydroxyalkanoates , Ecology , Hybridization , PCR-based methods",
author = "Diogo Queir{\'o}s and Lemos, {Paulo Alexandre da Costa} and Simona Rossetti and Serafim, {Lu{\'i}sa S.}",
note = "Sem PDF.",
year = "2015",
doi = "10.1007/s00253-015-7010-6",
language = "English",
volume = "99",
pages = "10433--10446",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Science Business Media",
number = "24",

}

Unveiling PHA-storing populations using molecular methods. / Queirós, Diogo; Lemos, Paulo Alexandre da Costa; Rossetti, Simona; Serafim, Luísa S.

In: Applied Microbiology and Biotechnology, Vol. 99, No. 24, 2015, p. 10433-10446.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Unveiling PHA-storing populations using molecular methods

AU - Queirós, Diogo

AU - Lemos, Paulo Alexandre da Costa

AU - Rossetti, Simona

AU - Serafim, Luísa S.

N1 - Sem PDF.

PY - 2015

Y1 - 2015

N2 - Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process.

AB - Enrichment of mixed microbial cultures (MMCs) in polyhydroxyalkanoate (PHA)-storing microorganisms must take place to develop a successful PHA production process. Moreover, throughout the operational period of a MMC system, the population needs to be checked in order to understand the changes in the performance that eventually occurred. For these reasons, it is necessary to monitor the population evolution, in order to identify the different groups of microorganisms and relate them with the storage capacity and kinetics of the MMC. Regarding this particular process, several culture-independent molecular techniques were already applied, with the use of hybridization techniques such fluorescence in situ hybridization and also PCR-based methods like denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism, pyrosequencing, and quantitative PCR standing out. This review intends, thus, to look at the molecular methods currently applied in monitoring the PHA-storing population evolution and how they can be combined with the evolutionary engineering step in order to optimize the overall process.

KW - Mixed microbial culture

KW - Polyhydroxyalkanoates

KW - Ecology

KW - Hybridization

KW - PCR-based methods

U2 - 10.1007/s00253-015-7010-6

DO - 10.1007/s00253-015-7010-6

M3 - Article

VL - 99

SP - 10433

EP - 10446

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 24

ER -