Unravelling the Effect of p,p′-Dichlorodiphenyldichloroethylene (DDE) in Hypertension of Wistar Rats

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Abstract

Hypertension is a multifactorial disease with limited knowledge of the involved mechanisms. p,p′-DDE (p,p′-dichlorodiphenyldichloroethylene) is a pollutant commonly found in tissues that interferes with endocrine signaling. This study aimed to evaluate the mechanism of hypertension triggered by p,p′-DDE exposure in the presence or absence of a HF (high-fat) diet in rats. The renin-angiotensin system (RAS) was evaluated by qPCR in liver and adipose tissue (AT), and a transcriptome analysis comparing visceral AT of HF diet and HF/DDE groups was performed. HF diet influenced RAS, but the p,p′-DDE effect was more evident in liver than in AT (interaction between the diet and p,p′-DDE treatment affected aldosterone receptor and angiotensin converting enzyme 2 expression in liver, p <0.05, two-way ANOVA). p,p′-DDE induced a decrease in the expression of genes involved in the retinoid acid biosynthesis pathway (Crabp1; -2.07-fold; p = 0.018), eNOS activation (Nos1; -1.64-fold; p = 0.012), and regulation and urea cycle (Ass1; -2.07-fold; p = 0.02). This study suggested that p,p′-DDE may play a fundamental role in the pathogenesis of hypertension, not exclusively in RAS but also by induction of hyperuricemia and increased oxidative stress, which may lead to endoplasmic reticulum stress and vascular injury. Copyright © 2018 American Chemical Society.
Original languageEnglish
Pages (from-to)12847-12854
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume66
Issue number48
DOIs
Publication statusPublished - Dec 2018

Fingerprint

Dichlorodiphenyl Dichloroethylene
renin-angiotensin system
high fat diet
hypertension
adipose tissue
Wistar Rats
Rats
Nutrition
Hypertension
liver
rats
Angiotensins
High Fat Diet
Renin-Angiotensin System
hyperuricemia
Fats
Renin
Liver
Tissue
retinoids

Keywords

  • adipose tissue
  • endocrine disruptor
  • high-fat
  • liver
  • renin-angiotensin system
  • Biochemistry
  • Cell membranes
  • Gene expression
  • Gene expression regulation
  • Liver
  • Polymerase chain reaction
  • Rats
  • Tissue
  • Urea
  • Adipose tissue
  • Angiotensin converting enzyme 2
  • Biosynthesis pathways
  • Endocrine disruptor
  • Endoplasmic reticulum stress
  • Multifactorial disease
  • Renin-angiotensin system
  • Transcriptome analysis
  • Chemical contamination

Cite this

@article{b28c2ec0a3084519b578bc5fcd214493,
title = "Unravelling the Effect of p,p′-Dichlorodiphenyldichloroethylene (DDE) in Hypertension of Wistar Rats",
abstract = "Hypertension is a multifactorial disease with limited knowledge of the involved mechanisms. p,p′-DDE (p,p′-dichlorodiphenyldichloroethylene) is a pollutant commonly found in tissues that interferes with endocrine signaling. This study aimed to evaluate the mechanism of hypertension triggered by p,p′-DDE exposure in the presence or absence of a HF (high-fat) diet in rats. The renin-angiotensin system (RAS) was evaluated by qPCR in liver and adipose tissue (AT), and a transcriptome analysis comparing visceral AT of HF diet and HF/DDE groups was performed. HF diet influenced RAS, but the p,p′-DDE effect was more evident in liver than in AT (interaction between the diet and p,p′-DDE treatment affected aldosterone receptor and angiotensin converting enzyme 2 expression in liver, p <0.05, two-way ANOVA). p,p′-DDE induced a decrease in the expression of genes involved in the retinoid acid biosynthesis pathway (Crabp1; -2.07-fold; p = 0.018), eNOS activation (Nos1; -1.64-fold; p = 0.012), and regulation and urea cycle (Ass1; -2.07-fold; p = 0.02). This study suggested that p,p′-DDE may play a fundamental role in the pathogenesis of hypertension, not exclusively in RAS but also by induction of hyperuricemia and increased oxidative stress, which may lead to endoplasmic reticulum stress and vascular injury. Copyright {\circledC} 2018 American Chemical Society.",
keywords = "adipose tissue, endocrine disruptor, high-fat, liver, renin-angiotensin system, Biochemistry, Cell membranes, Gene expression, Gene expression regulation, Liver, Polymerase chain reaction, Rats, Tissue, Urea, Adipose tissue, Angiotensin converting enzyme 2, Biosynthesis pathways, Endocrine disruptor, Endoplasmic reticulum stress, Multifactorial disease, Renin-angiotensin system, Transcriptome analysis, Chemical contamination",
author = "C. S{\'a} and D. Pestana and C. Calhau and A. Faria",
year = "2018",
month = "12",
doi = "10.1021/acs.jafc.8b05001",
language = "English",
volume = "66",
pages = "12847--12854",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "The American Chemical Society",
number = "48",

}

TY - JOUR

T1 - Unravelling the Effect of p,p′-Dichlorodiphenyldichloroethylene (DDE) in Hypertension of Wistar Rats

AU - Sá, C.

AU - Pestana, D.

AU - Calhau, C.

AU - Faria, A.

PY - 2018/12

Y1 - 2018/12

N2 - Hypertension is a multifactorial disease with limited knowledge of the involved mechanisms. p,p′-DDE (p,p′-dichlorodiphenyldichloroethylene) is a pollutant commonly found in tissues that interferes with endocrine signaling. This study aimed to evaluate the mechanism of hypertension triggered by p,p′-DDE exposure in the presence or absence of a HF (high-fat) diet in rats. The renin-angiotensin system (RAS) was evaluated by qPCR in liver and adipose tissue (AT), and a transcriptome analysis comparing visceral AT of HF diet and HF/DDE groups was performed. HF diet influenced RAS, but the p,p′-DDE effect was more evident in liver than in AT (interaction between the diet and p,p′-DDE treatment affected aldosterone receptor and angiotensin converting enzyme 2 expression in liver, p <0.05, two-way ANOVA). p,p′-DDE induced a decrease in the expression of genes involved in the retinoid acid biosynthesis pathway (Crabp1; -2.07-fold; p = 0.018), eNOS activation (Nos1; -1.64-fold; p = 0.012), and regulation and urea cycle (Ass1; -2.07-fold; p = 0.02). This study suggested that p,p′-DDE may play a fundamental role in the pathogenesis of hypertension, not exclusively in RAS but also by induction of hyperuricemia and increased oxidative stress, which may lead to endoplasmic reticulum stress and vascular injury. Copyright © 2018 American Chemical Society.

AB - Hypertension is a multifactorial disease with limited knowledge of the involved mechanisms. p,p′-DDE (p,p′-dichlorodiphenyldichloroethylene) is a pollutant commonly found in tissues that interferes with endocrine signaling. This study aimed to evaluate the mechanism of hypertension triggered by p,p′-DDE exposure in the presence or absence of a HF (high-fat) diet in rats. The renin-angiotensin system (RAS) was evaluated by qPCR in liver and adipose tissue (AT), and a transcriptome analysis comparing visceral AT of HF diet and HF/DDE groups was performed. HF diet influenced RAS, but the p,p′-DDE effect was more evident in liver than in AT (interaction between the diet and p,p′-DDE treatment affected aldosterone receptor and angiotensin converting enzyme 2 expression in liver, p <0.05, two-way ANOVA). p,p′-DDE induced a decrease in the expression of genes involved in the retinoid acid biosynthesis pathway (Crabp1; -2.07-fold; p = 0.018), eNOS activation (Nos1; -1.64-fold; p = 0.012), and regulation and urea cycle (Ass1; -2.07-fold; p = 0.02). This study suggested that p,p′-DDE may play a fundamental role in the pathogenesis of hypertension, not exclusively in RAS but also by induction of hyperuricemia and increased oxidative stress, which may lead to endoplasmic reticulum stress and vascular injury. Copyright © 2018 American Chemical Society.

KW - adipose tissue

KW - endocrine disruptor

KW - high-fat

KW - liver

KW - renin-angiotensin system

KW - Biochemistry

KW - Cell membranes

KW - Gene expression

KW - Gene expression regulation

KW - Liver

KW - Polymerase chain reaction

KW - Rats

KW - Tissue

KW - Urea

KW - Adipose tissue

KW - Angiotensin converting enzyme 2

KW - Biosynthesis pathways

KW - Endocrine disruptor

KW - Endoplasmic reticulum stress

KW - Multifactorial disease

KW - Renin-angiotensin system

KW - Transcriptome analysis

KW - Chemical contamination

U2 - 10.1021/acs.jafc.8b05001

DO - 10.1021/acs.jafc.8b05001

M3 - Article

VL - 66

SP - 12847

EP - 12854

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 48

ER -