@article{defcc431934a4325b1d9100875ff8011,
title = "Unraveling the multifaceted resilience of arsenic resistant bacterium Deinococcus indicus",
abstract = "Arsenic (As) is a toxic heavy metal widely found in the environment that severely undermines the integrity of water resources. Bioremediation of toxic compounds is an appellative sustainable technology with a balanced cost-effective setup. To pave the way for the potential use of Deinococcus indicus, an arsenic resistant bacterium, as a platform for arsenic bioremediation, an extensive characterization of its resistance to cellular insults is paramount. A comparative analysis of D. indicus cells grown in two rich nutrient media conditions (M53 and TGY) revealed distinct resistance patterns when cells are subjected to stress via UV-C and methyl viologen (MV). Cells grown in M53 demonstrated higher resistance to both UV-C and MV. Moreover, cells grow to higher density upon exposure to 25 mM As(V) in M53 in comparison with TGY. This analysis is pivotal for the culture of microbial species in batch culture bioreactors for bioremediation purposes. We also demonstrate for the first time the presence of polyphosphate granules in D. indicus which are also found in a few Deinococcus species. To extend our analysis, we also characterized DiArsC2 (arsenate reductase) involved in arsenic detoxification and structurally determined different states, revealing the structural evidence for a catalytic cysteine triple redox system. These results contribute for our understanding into the D. indicus resistance mechanism against stress conditions.",
keywords = "arsenate, arsenate reductase, metals, oxidative stress, PolyP granules, UV-C",
author = "Gouveia, {Andr{\'e} G.} and Salgueiro, {Bruno A.} and Ranmar, {Dean O.} and Antunes, {Wilson D.T.} and Peter Kirchweger and Ofra Golani and Wolf, {Sharon G.} and Michael Elbaum and Matias, {Pedro M.} and Rom{\~a}o, {C{\'e}lia V.}",
note = "Funding Information: This study was financially supported by the Portuguese Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologia (FCT), grants PTDC/BIA-BQM/31317/2017, Project MOSTMICRO-ITQB with references UIDB/04612/2020 and UIDP/04612/2020, and LS4FUTURE Associated Laboratory (LA/P/0087/2020). This project has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under grant agreement No. 857203. AG and BS are recipients of FCT grants SFRH/BD/06723/2020 and SFRH/BD/08066/2020, respectively. CR is recipient of FCT Institutional CEEC. Cryo-electron microscopy studies received partial support from the Weizmann Institute of Science (The Irving and Cherna Moskowitz Center for Nano and BioNano Imaging), and from the European Union (ERC-AdV grant, CryoSTEM, 101055413 to ME). This work benefited from access to the Weizmann Institute Electron Microscopy Unit, an Instruct-ERIC centre through the Access proposal PID: 19879. Funding Information: We would like to acknowledge the ALBA Synchrotron Light Facility with the collaboration of ALBA staff for the data collection of Di ArsC2 structures performed at BL13-XALOC beamline. The image analysis was made available thanks to the de Picciotto Cancer Cell Observatory In Memory of Wolfgang and Ruth Lesser of the MICC Life Sciences Core Facilities Weizmann Institute of Science Israel. Teresa Silva and Cristina Tim{\'o}teo from the Microbial Cell Production and Protein Purification and Characterization Research facilities at ITQB-NOVA are acknowledged for providing the competent cells of Escherichia coli strains and to purify TEV protease. Publisher Copyright: Copyright {\textcopyright} 2023 Gouveia, Salgueiro, Ranmar, Antunes, Kirchweger, Golani, Wolf, Elbaum, Matias and Rom{\~a}o.",
year = "2023",
doi = "10.3389/fmicb.2023.1240798",
language = "English",
volume = "14",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Research Foundation",
}