TY - JOUR
T1 - Ultrasound assisted extraction of cynaropicrin from Cynara cardunculus leaves: Optimization using the response surface methodology and the effect of pulse mode
AU - Brás, Teresa
AU - Paulino, Ana F. C.
AU - Neves, Luísa A.
AU - Crespo, João G.
AU - Duarte, Maria F.
N1 -
ALT20-03-0145-FEDER-000038
SFRH/BD/110969/2015
IF/00505/2014/CP1224/CT0004
UIDB/50006/2020
UIDB/05183/2020
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PY - 2020/8/1
Y1 - 2020/8/1
N2 - In this study, conditions for ultrasound assisted extraction (UAE) of cynaropicrin from Cynara cardunculus leaves by assessing the effect of pulsed mode and different duty cycles were optimized. Pulse mode presented a reduction of 45 % on energy consumption/ g cynaropicrin extracted, comparatively to continuous mode, and a duty cycle of 25 % presented the highest kinetic rate between the duty cycles under study, with a lower number of pulse repetitions necessary to reach a steady state conditions. The extraction methodology was further optimized by response surface methodology (RSM), using a Box-Bhenken design with 3 factors (solid/liquid ratio, amplitude and temperature), and 3 levels for correlation of independent variables with the extraction yield (mg of cynaropicrin/g dry weight) and cynaropicrin concentration (mg cynaropicrin /g extract). Experimental results were fitted to a second order polynomial model, using multiple regression and analysis of variance to determine the fitness of the model. Optimal conditions were found for a solid/liquid ratio of 1/27, amplitude of 67 % and temperature of 44 °C, with a predicted value of 23.99 mg/g DW and 192.23 mg/g extract for extraction yield and cynaropicrin concentration, respectively. The predicted values fit the experimental ones, with a 95 % confidence level showing the accuracy of the model. The results obtained suggest the applicability of the UAE optimization methodologies described, for cynaropicrin extraction from Cynara cardunculus leaves, illustrating its potential application on biotechnological and agro food industries.
AB - In this study, conditions for ultrasound assisted extraction (UAE) of cynaropicrin from Cynara cardunculus leaves by assessing the effect of pulsed mode and different duty cycles were optimized. Pulse mode presented a reduction of 45 % on energy consumption/ g cynaropicrin extracted, comparatively to continuous mode, and a duty cycle of 25 % presented the highest kinetic rate between the duty cycles under study, with a lower number of pulse repetitions necessary to reach a steady state conditions. The extraction methodology was further optimized by response surface methodology (RSM), using a Box-Bhenken design with 3 factors (solid/liquid ratio, amplitude and temperature), and 3 levels for correlation of independent variables with the extraction yield (mg of cynaropicrin/g dry weight) and cynaropicrin concentration (mg cynaropicrin /g extract). Experimental results were fitted to a second order polynomial model, using multiple regression and analysis of variance to determine the fitness of the model. Optimal conditions were found for a solid/liquid ratio of 1/27, amplitude of 67 % and temperature of 44 °C, with a predicted value of 23.99 mg/g DW and 192.23 mg/g extract for extraction yield and cynaropicrin concentration, respectively. The predicted values fit the experimental ones, with a 95 % confidence level showing the accuracy of the model. The results obtained suggest the applicability of the UAE optimization methodologies described, for cynaropicrin extraction from Cynara cardunculus leaves, illustrating its potential application on biotechnological and agro food industries.
KW - Cynara cardunculus
KW - Cynaropicrin
KW - Pulse mode
KW - Response surface methodology
KW - Ultrasound assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85082957347&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2020.112395
DO - 10.1016/j.indcrop.2020.112395
M3 - Article
AN - SCOPUS:85082957347
SN - 0926-6690
VL - 150
JO - Industrial Crops and Products
JF - Industrial Crops and Products
M1 - 112395
ER -