Ultrafiltration membrane cell recycle for continuous culture of Propionibacterium

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14 Citations (Scopus)

Abstract

A high-cell-concentration culture of Propionibacterium acidi-propionici in a fermenter with recycling through a tangential membrane filtration unit is described. Circulation rate and fermentation start-up procedures are discussed in order to optimize the system performance and the operation at high dilution rates. Maximum cell concentrations up to 140 g dry weight/l were obtained, with a propionic acid productivity of 10.3 g/l hr and a product yield of 0.56 g propionic acid/g glucose. Rheological studies showed that at cell mass concentrations above 90-100 g/1 the fermentation broth viscosity increased rapidly and changed its behaviour from Newtonian to pseudoplastic. The use of an adequate cell bleed rate allowed the cell concentration to be maintained at a desirable value and consequently to stabilize the reactor operation.

Original languageEnglish
Pages (from-to)303-314
Number of pages12
JournalJournal of Membrane Science
Volume61
Issue numberC
DOIs
Publication statusPublished - 1991

Fingerprint

Propionibacterium
Propionic acid
Ultrafiltration
Cell membranes
Cell culture
Fermentation
Cell Membrane
Fermenters
Reactor operation
propionic acid
cells
fermentation
Dilution
Glucose
Recycling
Productivity
Viscosity
Membranes
broths
recycling

Keywords

  • cell recycling
  • continuous culture
  • Propionibacterium acidi-propionici
  • propionic acid
  • ultrafiltration membrane

Cite this

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title = "Ultrafiltration membrane cell recycle for continuous culture of Propionibacterium",
abstract = "A high-cell-concentration culture of Propionibacterium acidi-propionici in a fermenter with recycling through a tangential membrane filtration unit is described. Circulation rate and fermentation start-up procedures are discussed in order to optimize the system performance and the operation at high dilution rates. Maximum cell concentrations up to 140 g dry weight/l were obtained, with a propionic acid productivity of 10.3 g/l hr and a product yield of 0.56 g propionic acid/g glucose. Rheological studies showed that at cell mass concentrations above 90-100 g/1 the fermentation broth viscosity increased rapidly and changed its behaviour from Newtonian to pseudoplastic. The use of an adequate cell bleed rate allowed the cell concentration to be maintained at a desirable value and consequently to stabilize the reactor operation.",
keywords = "cell recycling, continuous culture, Propionibacterium acidi-propionici, propionic acid, ultrafiltration membrane",
author = "Crespo, {J. P. S. G.} and Moura, {M. J.} and Almeida, {J. S.} and Carrondo, {M. J. T.}",
note = "We acknowledge the financial support of Junta National de Investigacao Cientifica e Tecnologica, Lisboa (JNICT) under contract No. 720235.74 and Stiftung Volkswagenwerk. M.J. Moura acknowledges a research grant awarded by JNICT. We hereby express our recognition for the ongoing collaborations with Prof. Gerard Goma (INSA, Toulouse, France) and Prof. Karl Schugerl (TCI, Hannover, Germany).",
year = "1991",
doi = "10.1016/0376-7388(91)80023-Y",
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journal = "Journal of Membrane Science",
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TY - JOUR

T1 - Ultrafiltration membrane cell recycle for continuous culture of Propionibacterium

AU - Crespo, J. P. S. G.

AU - Moura, M. J.

AU - Almeida, J. S.

AU - Carrondo, M. J. T.

N1 - We acknowledge the financial support of Junta National de Investigacao Cientifica e Tecnologica, Lisboa (JNICT) under contract No. 720235.74 and Stiftung Volkswagenwerk. M.J. Moura acknowledges a research grant awarded by JNICT. We hereby express our recognition for the ongoing collaborations with Prof. Gerard Goma (INSA, Toulouse, France) and Prof. Karl Schugerl (TCI, Hannover, Germany).

PY - 1991

Y1 - 1991

N2 - A high-cell-concentration culture of Propionibacterium acidi-propionici in a fermenter with recycling through a tangential membrane filtration unit is described. Circulation rate and fermentation start-up procedures are discussed in order to optimize the system performance and the operation at high dilution rates. Maximum cell concentrations up to 140 g dry weight/l were obtained, with a propionic acid productivity of 10.3 g/l hr and a product yield of 0.56 g propionic acid/g glucose. Rheological studies showed that at cell mass concentrations above 90-100 g/1 the fermentation broth viscosity increased rapidly and changed its behaviour from Newtonian to pseudoplastic. The use of an adequate cell bleed rate allowed the cell concentration to be maintained at a desirable value and consequently to stabilize the reactor operation.

AB - A high-cell-concentration culture of Propionibacterium acidi-propionici in a fermenter with recycling through a tangential membrane filtration unit is described. Circulation rate and fermentation start-up procedures are discussed in order to optimize the system performance and the operation at high dilution rates. Maximum cell concentrations up to 140 g dry weight/l were obtained, with a propionic acid productivity of 10.3 g/l hr and a product yield of 0.56 g propionic acid/g glucose. Rheological studies showed that at cell mass concentrations above 90-100 g/1 the fermentation broth viscosity increased rapidly and changed its behaviour from Newtonian to pseudoplastic. The use of an adequate cell bleed rate allowed the cell concentration to be maintained at a desirable value and consequently to stabilize the reactor operation.

KW - cell recycling

KW - continuous culture

KW - Propionibacterium acidi-propionici

KW - propionic acid

KW - ultrafiltration membrane

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U2 - 10.1016/0376-7388(91)80023-Y

DO - 10.1016/0376-7388(91)80023-Y

M3 - Article

VL - 61

SP - 303

EP - 314

JO - Journal of Membrane Science

JF - Journal of Membrane Science

SN - 0376-7388

IS - C

ER -