TY - JOUR
T1 - Trypanosoma cruzi strain TcI is associated with chronic Chagas disease in the Brazilian Amazon
AU - Santana, Rosa Amélia Gonçalves
AU - Magalhães, Laylah Kelre Costa
AU - Magalhães, Laise Kelman Costa
AU - Prestes, Suzane Ribeiro
AU - Maciel, Marcel Gonçalves
AU - Da Silva, George Allan Villarouco
AU - Monteiro, Wuelton Marcelo
AU - De Brito, Felipe Rocha
AU - De Aguiar Raposo Câmara Coelho, Leila Inês
AU - Barbosa-Ferreira, João Marcos
AU - Guerra, Jorge Augusto Oliveira
AU - Silveira, Henrique
AU - Das Graças Vale Barbosa, Maria
N1 - PMID:24916362
WOS:000338361500001
PY - 2014/6/11
Y1 - 2014/6/11
N2 - Background: Chagas disease in the Amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (DTUs) TcI and TcIV. These DTUs are responsible for cases of acute disease associated with oral transmission. Chronic disease cases have been detected through serological surveys. However, the mode of transmission could not be determined, or any association of chronic disease with a specific T. cruzi DTU's. The aim of this study was to characterize Trypanosoma cruzi in patients with chronic Chagas disease in the State of Amazonas, Brazil. Methods. Blood culture and xenodiagnosis were performed in 36 patients with positive serology for Chagas disease who participated in a serological survey performed in urban and rural areas of Manaus, Amazonas. DNA samples were extracted from the feces of triatomines used for xenodiagnosis, and the nontranscribed spacer of the mini-exon gene and the mitochondrial gene cytochrome oxidase subunit II (COII) were amplified by PCR and sequenced. Results: Blood culture and xenodiagnosis were negative in 100% of samples; however, molecular techniques revealed that in 13 out of 36 (36%) fecal samples from xenodiagnosis, T. cruzi was characterized as the DTU TcI, and different haplotypes were identified within the same DTU. Conclusion: The DTU TcI, which is mainly associated with acute cases of Chagas disease in the Amazon region, is also responsible for chronic infection in patients from a region in the State of Amazonas.
AB - Background: Chagas disease in the Amazon region is considered an emerging anthropozoonosis with a predominance of the discrete typing units (DTUs) TcI and TcIV. These DTUs are responsible for cases of acute disease associated with oral transmission. Chronic disease cases have been detected through serological surveys. However, the mode of transmission could not be determined, or any association of chronic disease with a specific T. cruzi DTU's. The aim of this study was to characterize Trypanosoma cruzi in patients with chronic Chagas disease in the State of Amazonas, Brazil. Methods. Blood culture and xenodiagnosis were performed in 36 patients with positive serology for Chagas disease who participated in a serological survey performed in urban and rural areas of Manaus, Amazonas. DNA samples were extracted from the feces of triatomines used for xenodiagnosis, and the nontranscribed spacer of the mini-exon gene and the mitochondrial gene cytochrome oxidase subunit II (COII) were amplified by PCR and sequenced. Results: Blood culture and xenodiagnosis were negative in 100% of samples; however, molecular techniques revealed that in 13 out of 36 (36%) fecal samples from xenodiagnosis, T. cruzi was characterized as the DTU TcI, and different haplotypes were identified within the same DTU. Conclusion: The DTU TcI, which is mainly associated with acute cases of Chagas disease in the Amazon region, is also responsible for chronic infection in patients from a region in the State of Amazonas.
UR - http://www.scopus.com/inward/record.url?scp=84903783842&partnerID=8YFLogxK
U2 - 10.1186/1756-3305-7-267
DO - 10.1186/1756-3305-7-267
M3 - Article
C2 - 24916362
AN - SCOPUS:84903783842
VL - 7
JO - Parasites & Vectors
JF - Parasites & Vectors
IS - 1
M1 - 267
ER -
