Transthyretin amyloidosis

Chaperone concentration changes and increased proteolysis in the pathway to disease

Gonçalo Da Costa, Cristina Ribeiro-Silva, Raquel Ribeiro, Samuel Gilberto, Ricardo A. Gomes, António Ferreira, Élia Mateus, Eduardo Barroso, Ana Maria Coelho, Ana Ponces Freire, Carlos Cordeiro

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Transthyretin amyloidosis is a conformational pathology characterized by the extracellular formation of amyloid deposits and the progressive impairment of the peripheral nervous system. Point mutations in this tetrameric plasma protein decrease its stability and are linked to disease onset and progression. Since non-mutated transthyretin also forms amyloid in systemic senile amyloidosis and some mutation bearers are asymptomatic throughout their lives, non-genetic factors must also be involved in transthyretin amyloidosis. We discovered, using a differential proteomics approach, that extracellular chaperones such as fibrinogen, clusterin, haptoglobin, alpha-1-anti-trypsin and 2-macroglobulin are overrepresented in transthyretin amyloidosis. Our data shows that a complex network of extracellular chaperones are over represented in human plasma and we speculate that they act synergistically to cope with amyloid prone proteins. Proteostasis may thus be as important as point mutations in transthyretin amyloidosis.

Original languageEnglish
Article numbere0125392
JournalPlosOne
Volume10
Issue number7
DOIs
Publication statusPublished - 6 Jul 2015

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Proteolysis
amyloidosis
prealbumin
Prealbumin
proteolysis
amyloid
Point Mutation
point mutation
Amyloid
Clusterin
Amyloidogenic Proteins
Macroglobulins
Haptoglobins
Amyloid Plaques
Peripheral Nervous System
macroglobulins
Plasma (human)
Amyloidosis
peripheral nervous system
Proteomics

Cite this

Da Costa, G., Ribeiro-Silva, C., Ribeiro, R., Gilberto, S., Gomes, R. A., Ferreira, A., ... Cordeiro, C. (2015). Transthyretin amyloidosis: Chaperone concentration changes and increased proteolysis in the pathway to disease. PlosOne, 10(7), [e0125392]. https://doi.org/10.1371/journal.pone.0125392
Da Costa, Gonçalo ; Ribeiro-Silva, Cristina ; Ribeiro, Raquel ; Gilberto, Samuel ; Gomes, Ricardo A. ; Ferreira, António ; Mateus, Élia ; Barroso, Eduardo ; Coelho, Ana Maria ; Freire, Ana Ponces ; Cordeiro, Carlos. / Transthyretin amyloidosis : Chaperone concentration changes and increased proteolysis in the pathway to disease. In: PlosOne. 2015 ; Vol. 10, No. 7.
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Da Costa, G, Ribeiro-Silva, C, Ribeiro, R, Gilberto, S, Gomes, RA, Ferreira, A, Mateus, É, Barroso, E, Coelho, AM, Freire, AP & Cordeiro, C 2015, 'Transthyretin amyloidosis: Chaperone concentration changes and increased proteolysis in the pathway to disease', PlosOne, vol. 10, no. 7, e0125392. https://doi.org/10.1371/journal.pone.0125392

Transthyretin amyloidosis : Chaperone concentration changes and increased proteolysis in the pathway to disease. / Da Costa, Gonçalo; Ribeiro-Silva, Cristina; Ribeiro, Raquel; Gilberto, Samuel; Gomes, Ricardo A.; Ferreira, António; Mateus, Élia; Barroso, Eduardo; Coelho, Ana Maria; Freire, Ana Ponces; Cordeiro, Carlos.

In: PlosOne, Vol. 10, No. 7, e0125392, 06.07.2015.

Research output: Contribution to journalArticle

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AU - Freire, Ana Ponces

AU - Cordeiro, Carlos

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Da Costa G, Ribeiro-Silva C, Ribeiro R, Gilberto S, Gomes RA, Ferreira A et al. Transthyretin amyloidosis: Chaperone concentration changes and increased proteolysis in the pathway to disease. PlosOne. 2015 Jul 6;10(7). e0125392. https://doi.org/10.1371/journal.pone.0125392