Transcriptomics and physiological analyses reveal co-ordinated alteration of metabolic pathways in Jatropha curcas drought tolerance

Helena Sapeta, Tiago Lourenço, Stefan Lorenz, Christian Grumaz, Philipp Kirstahler, Pedro M. Barros, Joaquim Miguel Costa, Kai Sohn, M. Margarida Oliveira

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Jatropha curcas, a multipurpose plant attracting a great deal of attention due to its high oil content and quality for biofuel, is recognized as a drought-tolerant species. However, this drought tolerance is still poorly characterized. This study aims to contribute to uncover the molecular background of this tolerance, using a combined approach of transcriptional profiling and morphophysiological characterization during a period of water-withholding (49 d) followed by rewatering (7 d). Morphophysiological measurements showed that J. curcas plants present different adaptation strategies to withstand moderate and severe drought. Therefore, RNA sequencing was performed for samples collected under moderate and severe stress followed by rewatering, for both roots and leaves. Jatropha curcas transcriptomic analysis revealed shoot- and root-specific adaptations across all investigated conditions, except under severe stress, when the dramatic transcriptomic reorganization at the root and shoot level surpassed organ specificity. These changes in gene expression were clearly shown by the down-regulation of genes involved in growth and water uptake, and up-regulation of genes related to osmotic adjustments and cellular homeostasis. However, organ-specific gene variations were also detected, such as strong up-regulation of abscisic acid synthesis in roots under moderate stress and of chlorophyll metabolism in leaves under severe stress. Functional validation further corroborated the differential expression of genes coding for enzymes involved in chlorophyll metabolism, which correlates with the metabolite content of this pathway.

Original languageEnglish
Pages (from-to)845-860
Number of pages16
JournalJournal Of Experimental Botany
Volume67
Issue number3
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • Chlorophylls
  • gene profiling
  • Jatropha curcas
  • leaf and root
  • leaf gas exchange
  • morphology
  • progressive drought
  • purging nut, RNA-Seq
  • water stress-recovery cycle

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