Transcriptional Response of Human Neurospheres to Helper-Dependent CAV-2 Vectors Involves the Modulation of DNA Damage Response, Microtubule and Centromere Gene Groups

Stefania Piersanti, Romina Burla, Valerio Licursi, Ana Catarina Montes, Mattia La Torre, Paula M. Alves, Daniel Simao, Carla Mottini, Sara Salinas, Rodolfo Negri, Enrico Tagliafico, Eric J. Kremer, Isabella Saggio

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Brain gene transfer using viral vectors will likely become a therapeutic option for several disorders. Helper-dependent (HD) canine adenovirus type 2 vectors (CAV-2) are well suited for this goal. These vectors are poorly immunogenic, efficiently transduce neurons, are retrogradely transported to afferent structures in the brain and lead to long-term transgene expression. CAV-2 vectors are being exploited to unravel behavior, cognition, neural networks, axonal transport and therapy for orphan diseases. With the goal of better understanding and characterizing HD-CAV-2 for brain therapy, we analyzed the transcriptomic modulation induced by HD-CAV-2 in human differentiated neurospheres derived from mid-brain progenitors. This 3D model system mimics several aspects of the dynamic nature of human brain. We found that differentiated neurospheres are readily transduced by HD-CAV- 2 and that transduction generates two main transcriptional responses: a DNA damage response and alteration of centromeric and microtubule probes. Future investigations on the biochemistry of processes highlighted by probe modulations will help defining the implication of HD-CAV-2 and CAR receptor binding in enchaining these functional pathways. We suggest here that the modulation of DNA damage genes is related to viral DNA, while the alteration of centromeric and microtubule probes is possibly enchained by the interaction of the HD-CAV-2 fibre with CAR.

Original languageEnglish
Article number0133607
Number of pages17
JournalPlosOne
Volume10
Issue number7
DOIs
Publication statusPublished - 24 Jul 2015

Keywords

  • CANINE ADENOVIRUS TYPE-2
  • CELLULAR TRANSCRIPTION
  • IN-VIVO
  • CENP-A
  • RECEPTOR
  • COMPLEX
  • INFECTION
  • CELLS
  • COXSACKIEVIRUS
  • TRANSDUCTION

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