Tracing the origin of an outbreak of methicillin-resistant Staphylococcus aureus infections in a Portuguese hospital by molecular fingerprinting methods

I. Santos Sanches, M. Aires De Sousa, L. Cleto, M. Baeta de Campos, H. De Lencastre

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Seventy-six methicillin-resistant Staphylococcus aureus (MRSA) isolates were collected from July 1992 to May 1995 at a 400-bed district hospital in the northeast of Portugal. During the second half of the surveillance period, in July of 1994, an outbreak was detected in the orthopedic ward. Thirty- three (out of the 76) MRSA strains were recovered only in this ward during the outbreak period. All strains were characterized by a variety of genomic fingerprints. Hybridization of ClaI and SmaI restriction digests with the mecA- and Tn554-specific DNA probes was used to identify polymorphism and determine chromosomal location of these determinants, and pulsed-field gel electrophoretic analysis of SmaI digests was used to determine chromosomal backgrounds. All strains recovered during the outbreak in the orthopedic ward were found to belong to a single clone that carried the mecA polymorph I, Tn554 type E in a macrorestriction background called H (clone I::E::H1), which was identified in 18 patients, and 5 health care personnel and from a fomite sample, and was traced to a single transfer patient admitted to the hospital at the beginning of the outbreak. The new clone I::E::H1 differed only in the macrorestriction profile from the MRSA clone previously dominant in this hospital, known as Iberian epidemic clone I::E::A, which has already been identified in several Spanish and Portuguese hospitals.

Original languageEnglish
Pages (from-to)319-329
Number of pages11
JournalMicrobial Drug Resistance
Volume2
Issue number3
DOIs
Publication statusPublished - 22 Sep 1996

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