Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.)

Douglas Jardim-Messeder, Andréia Caverzan, Rafael Rauber, Juliana R. Cunha, Fabrício E.L. Carvalho, Marcos Letaif Gaeta, Guilherme Cordenonsi da Fonseca, J. Miguel Costa, Michael Frei, Joaquim A.G. Silveira, Rogério Margis, Nelson J.M. Saibo, Marcia Margis-Pinheiro

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In rice (Oryza sativa L.) there are two chloroplast ascorbate peroxidases isoforms, OsAPX7 (stromal) and OsAPX8 (thylakoidal). Previous works demonstrated that double silencing of OsAPX7 and OsAPX8 led to differences in photosynthetic parameters in stress conditions. Nevertheless, both APX encoding genes present contrasting expression patterns in response to abiotic stress and the individual role of them in chloroplast redox metabolism and in stress response remains largely unknown. Here, we showed that plants silenced to OsAPX8 (RNAiOsAPX8) have a lower growth rate and a higher H2O2 content, when compared to non-transformed (NT) plants. Furthermore, RNAiOsAPX8 plants show a decrease in stomatal opening with consequent increase in leaf surface temperature. However, these plants were not more susceptible to drought stress. RNAseq analysis showed that the silencing of OsAPX8 has impact on plant gene expression, mainly affecting genes related to photosynthesis and signal transduction. Our results indicate that the reduction of OsAPX8 expression led to a stress-like phenotype but it does not alter drought tolerance.

Original languageEnglish
Pages (from-to)46-56
Number of pages11
JournalEnvironmental and Experimental Botany
Volume150
DOIs
Publication statusPublished - 1 Jun 2018

Fingerprint

hydrogen peroxide
Oryza sativa
rice
chloroplast
chloroplasts
gene
drought stress
ascorbate peroxidase
drought tolerance
abiotic stress
gene expression
surface temperature
signal transduction
phenotype
stress response
photosynthesis
water stress
genes
tolerance
metabolism

Keywords

  • Ascorbate peroxidase
  • Chloroplast
  • Photosynthesis
  • Reactive oxygen species
  • Stomatal closure
  • Stress response

Cite this

Jardim-Messeder, D., Caverzan, A., Rauber, R., Cunha, J. R., Carvalho, F. E. L., Gaeta, M. L., ... Margis-Pinheiro, M. (2018). Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.). Environmental and Experimental Botany, 150, 46-56. https://doi.org/10.1016/j.envexpbot.2018.02.012
Jardim-Messeder, Douglas ; Caverzan, Andréia ; Rauber, Rafael ; Cunha, Juliana R. ; Carvalho, Fabrício E.L. ; Gaeta, Marcos Letaif ; da Fonseca, Guilherme Cordenonsi ; Costa, J. Miguel ; Frei, Michael ; Silveira, Joaquim A.G. ; Margis, Rogério ; Saibo, Nelson J.M. ; Margis-Pinheiro, Marcia. / Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.). In: Environmental and Experimental Botany. 2018 ; Vol. 150. pp. 46-56.
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title = "Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.)",
abstract = "In rice (Oryza sativa L.) there are two chloroplast ascorbate peroxidases isoforms, OsAPX7 (stromal) and OsAPX8 (thylakoidal). Previous works demonstrated that double silencing of OsAPX7 and OsAPX8 led to differences in photosynthetic parameters in stress conditions. Nevertheless, both APX encoding genes present contrasting expression patterns in response to abiotic stress and the individual role of them in chloroplast redox metabolism and in stress response remains largely unknown. Here, we showed that plants silenced to OsAPX8 (RNAiOsAPX8) have a lower growth rate and a higher H2O2 content, when compared to non-transformed (NT) plants. Furthermore, RNAiOsAPX8 plants show a decrease in stomatal opening with consequent increase in leaf surface temperature. However, these plants were not more susceptible to drought stress. RNAseq analysis showed that the silencing of OsAPX8 has impact on plant gene expression, mainly affecting genes related to photosynthesis and signal transduction. Our results indicate that the reduction of OsAPX8 expression led to a stress-like phenotype but it does not alter drought tolerance.",
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Jardim-Messeder, D, Caverzan, A, Rauber, R, Cunha, JR, Carvalho, FEL, Gaeta, ML, da Fonseca, GC, Costa, JM, Frei, M, Silveira, JAG, Margis, R, Saibo, NJM & Margis-Pinheiro, M 2018, 'Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.)', Environmental and Experimental Botany, vol. 150, pp. 46-56. https://doi.org/10.1016/j.envexpbot.2018.02.012

Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.). / Jardim-Messeder, Douglas; Caverzan, Andréia; Rauber, Rafael; Cunha, Juliana R.; Carvalho, Fabrício E.L.; Gaeta, Marcos Letaif; da Fonseca, Guilherme Cordenonsi; Costa, J. Miguel; Frei, Michael; Silveira, Joaquim A.G.; Margis, Rogério; Saibo, Nelson J.M.; Margis-Pinheiro, Marcia.

In: Environmental and Experimental Botany, Vol. 150, 01.06.2018, p. 46-56.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Thylakoidal APX modulates hydrogen peroxide content and stomatal closure in rice (Oryza sativa L.)

AU - Jardim-Messeder, Douglas

AU - Caverzan, Andréia

AU - Rauber, Rafael

AU - Cunha, Juliana R.

AU - Carvalho, Fabrício E.L.

AU - Gaeta, Marcos Letaif

AU - da Fonseca, Guilherme Cordenonsi

AU - Costa, J. Miguel

AU - Frei, Michael

AU - Silveira, Joaquim A.G.

AU - Margis, Rogério

AU - Saibo, Nelson J.M.

AU - Margis-Pinheiro, Marcia

PY - 2018/6/1

Y1 - 2018/6/1

N2 - In rice (Oryza sativa L.) there are two chloroplast ascorbate peroxidases isoforms, OsAPX7 (stromal) and OsAPX8 (thylakoidal). Previous works demonstrated that double silencing of OsAPX7 and OsAPX8 led to differences in photosynthetic parameters in stress conditions. Nevertheless, both APX encoding genes present contrasting expression patterns in response to abiotic stress and the individual role of them in chloroplast redox metabolism and in stress response remains largely unknown. Here, we showed that plants silenced to OsAPX8 (RNAiOsAPX8) have a lower growth rate and a higher H2O2 content, when compared to non-transformed (NT) plants. Furthermore, RNAiOsAPX8 plants show a decrease in stomatal opening with consequent increase in leaf surface temperature. However, these plants were not more susceptible to drought stress. RNAseq analysis showed that the silencing of OsAPX8 has impact on plant gene expression, mainly affecting genes related to photosynthesis and signal transduction. Our results indicate that the reduction of OsAPX8 expression led to a stress-like phenotype but it does not alter drought tolerance.

AB - In rice (Oryza sativa L.) there are two chloroplast ascorbate peroxidases isoforms, OsAPX7 (stromal) and OsAPX8 (thylakoidal). Previous works demonstrated that double silencing of OsAPX7 and OsAPX8 led to differences in photosynthetic parameters in stress conditions. Nevertheless, both APX encoding genes present contrasting expression patterns in response to abiotic stress and the individual role of them in chloroplast redox metabolism and in stress response remains largely unknown. Here, we showed that plants silenced to OsAPX8 (RNAiOsAPX8) have a lower growth rate and a higher H2O2 content, when compared to non-transformed (NT) plants. Furthermore, RNAiOsAPX8 plants show a decrease in stomatal opening with consequent increase in leaf surface temperature. However, these plants were not more susceptible to drought stress. RNAseq analysis showed that the silencing of OsAPX8 has impact on plant gene expression, mainly affecting genes related to photosynthesis and signal transduction. Our results indicate that the reduction of OsAPX8 expression led to a stress-like phenotype but it does not alter drought tolerance.

KW - Ascorbate peroxidase

KW - Chloroplast

KW - Photosynthesis

KW - Reactive oxygen species

KW - Stomatal closure

KW - Stress response

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