Thermal and chemical unfolding studies in chemotaxis heme sensors from Geobacter sulfurreducens

Carlos Alberto Gomes Salgueiro

Thermal and chemical unfolding studies in chemotaxis heme sensors from Geobacter sulfurreducens

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The thermal and chemical unfolding mechanisms of periplasmic hemic sensor domains GSU0582 and GSU0935 were investigated by far-UV CD and UV-visible spectra. The sensors have a reversible two-state cooperative unfolding mechanism, with different T m, C m and conformation stability parameters, suggesting that GSU0582 is conformationally more stable. These findings and differences in the dynamic properties of the two sensors might provide clues into dimer assembly.

Original language	Unknown
Title of host publication	10TH EUROPEAN BIOLOGICAL INORGANIC CHEMISTRY CONFERENCE (EUROBIC10)
Pages	43-45
Publication status	Published - 1 Jan 2010
Event	10th European Biological Inorganic Chemistry Conference (EUROBIC10) - Duration: 1 Jan 2010 → …

Conference

Conference	10th European Biological Inorganic Chemistry Conference (EUROBIC10)
Period	1/01/10 → …

Cite this

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title = "Thermal and chemical unfolding studies in chemotaxis heme sensors from Geobacter sulfurreducens",

abstract = "The thermal and chemical unfolding mechanisms of periplasmic hemic sensor domains GSU0582 and GSU0935 were investigated by far-UV CD and UV-visible spectra. The sensors have a reversible two-state cooperative unfolding mechanism, with different T m, C m and conformation stability parameters, suggesting that GSU0582 is conformationally more stable. These findings and differences in the dynamic properties of the two sensors might provide clues into dimer assembly.",

author = "Salgueiro, {Carlos Alberto Gomes}",

year = "2010",

month = jan,

day = "1",

language = "Unknown",

isbn = "978-88-7587-592-3",

pages = "43--45",

booktitle = "10TH EUROPEAN BIOLOGICAL INORGANIC CHEMISTRY CONFERENCE (EUROBIC10)",

note = "10th European Biological Inorganic Chemistry Conference (EUROBIC10) ; Conference date: 01-01-2010",

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T1 - Thermal and chemical unfolding studies in chemotaxis heme sensors from Geobacter sulfurreducens

AU - Salgueiro, Carlos Alberto Gomes

PY - 2010/1/1

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N2 - The thermal and chemical unfolding mechanisms of periplasmic hemic sensor domains GSU0582 and GSU0935 were investigated by far-UV CD and UV-visible spectra. The sensors have a reversible two-state cooperative unfolding mechanism, with different T m, C m and conformation stability parameters, suggesting that GSU0582 is conformationally more stable. These findings and differences in the dynamic properties of the two sensors might provide clues into dimer assembly.

AB - The thermal and chemical unfolding mechanisms of periplasmic hemic sensor domains GSU0582 and GSU0935 were investigated by far-UV CD and UV-visible spectra. The sensors have a reversible two-state cooperative unfolding mechanism, with different T m, C m and conformation stability parameters, suggesting that GSU0582 is conformationally more stable. These findings and differences in the dynamic properties of the two sensors might provide clues into dimer assembly.

M3 - Conference contribution

SN - 978-88-7587-592-3

SP - 43

EP - 45

BT - 10TH EUROPEAN BIOLOGICAL INORGANIC CHEMISTRY CONFERENCE (EUROBIC10)

T2 - 10th European Biological Inorganic Chemistry Conference (EUROBIC10)

Y2 - 1 January 2010

ER -