The thermal and chemical unfolding mechanisms of periplasmic hemic sensor domains GSU0582 and GSU0935 were investigated by far-UV CD and UV-visible spectra. The sensors have a reversible two-state cooperative unfolding mechanism, with different T m, C m and conformation stability parameters, suggesting that GSU0582 is conformationally more stable. These findings and differences in the dynamic properties of the two sensors might provide clues into dimer assembly.
|Title of host publication||10TH EUROPEAN BIOLOGICAL INORGANIC CHEMISTRY CONFERENCE (EUROBIC10)|
|Publication status||Published - 1 Jan 2010|
|Event||10th European Biological Inorganic Chemistry Conference (EUROBIC10) - |
Duration: 1 Jan 2010 → …
|Conference||10th European Biological Inorganic Chemistry Conference (EUROBIC10)|
|Period||1/01/10 → …|