To study the role of b5 on the activity of human CYP, we developed human b5/CYP competent bacteria containing a bi-plasmid system for the co-expression of a specific CYP form (CYP1A2, 2A6 or 2E1) with human b5 and human NADPH cytochrome P450 reductase (RED). The relative content of b5 with CYP and RED of these new strains demonstrated physiologically relevant co-expressing levels. These new strains demonstrated a strong stimulatory effect of b5 on CYP2A6 and 2E1 bioactivation activities which was less pronounced for CYP1A2.
|Title of host publication||Proceedings of the 14th International Conference on Cytochromes P450: Biochemistry, Biophysics, and Bioinformatics|
|Publication status||Published - 1 Jan 2005|
|Event||14th International Conference on Cytochromes P450 - Biochemistry, Biophysics, and Bioinformatics Location: Dallas, TX - |
Duration: 1 Jan 2005 → …
|Conference||14th International Conference on Cytochromes P450 - Biochemistry, Biophysics, and Bioinformatics Location: Dallas, TX|
|Period||1/01/05 → …|