TY - JOUR
T1 - The role of muscle in the susceptibility and progression of axial Spondyloarthritis
T2 - The MyoSpA Study Protocol
AU - Sardoo, Atlas Mashayekhi
AU - Neto, Agna
AU - Pinheiro Torres, Rita
AU - Rodrigues-Manica, Santiago
AU - Domingues, Lúcia
AU - Lage Crespo, Carolina
AU - Lagoas-Gomes, João
AU - Mascarenhas, Vasco
AU - Mendes, César S
AU - Galzerano, Antonio
AU - Fernandes de Almeida, Sérgio
AU - Sepriano, Alexandre
AU - Ramiro, Sofia
AU - Masi, Alfonse T
AU - Nair, Kalyani
AU - Costa, Julia
AU - Alexandre, Bruno Miguel
AU - Vassilevskaia, Tatiana
AU - Cunha, Celso Vladimiro
AU - Sobral, Daniel
AU - Branco, Jaime Cunha
AU - Gomes-Alves, Patrícia
AU - Pimentel-Santos, Fernando M
N1 - declArAtions
ETHICS APPROVAL AND CONSENT TO PARTICIPATE
The current study was submitted and approved by the ethical committee of University of Lisbon and Centro Hospitalar de Lisboa Ocidental, Hospital de Egas Moniz, EPE (Reference Number: 20170700050). The study will be conducted in accordance with the International Conference on Harmonization Good Clinical Practice (GCP) and the Declaration of Helsinki. Furthermore, voluntary written informed participants’ consent will be obtained from all subjects before the start of the study procedures.
FUNDING
This study was supported by iNOVA4Health (consortia to create a multidisdiplinary/translational network at the NOVA University, Lisbon, Portugal) and Portuguese Society of Rheumatology grants.
PY - 2021/12/29
Y1 - 2021/12/29
N2 - BACKGROUND: Axial Spondyloarthritis (axSpA) is a chronic, inflammatory rheumatic disease that affects the axial skeleton, causing pain, stiffness, and fatigue. Genetics and environmental factors such as microbiota and microtrauma are known causes of disease susceptibility and progression. Murine models of axSpA found a decisive role for biomechanical stress as an inducer of enthesitis and new bone formation. Here, we hypothesize that muscle properties in axSpA patients are compromised and influenced by genetic background.OBJECTIVES: To improve our current knowledge of axSpA physiopathology, we aim to characterize axial and peripheral muscle properties and identify genetic and protein biomarker that might explain such properties.METHODS: A cross-sectional study will be conducted on 48 participants aged 18-50 years old, involving patients with axSpA (according to ASAS classification criteria, symptoms duration < 10 years) and healthy controls matched by gender, age, and levels of physical activity. We will collect epidemiological and clinical data and perform a detailed, whole body and segmental, myofascial characterization (focusing on multifidus, brachioradialis and the gastrocnemius lateralis) concerning: a) Physical Properties (stiffness, tone and elasticity), assessed by MyotonPRO®; b) Strength, by a dynamometer; c) Mass, by bioimpedance; d) Performance through gait speed and 60-second sit-to-stand test; e) Histological and cellular/ molecular characterization through ultrasound-guided biopsies of multifidus muscle; f) Magnetic Resonance Imaging (MRI) characterization of paravertebral muscles. Furthermore, we will perform an integrated transcriptomics and proteomics analysis of peripheral blood samples.DISCUSSION: The innovative and multidisciplinary approaches of this project rely on the elucidation of myofascial physical properties in axSpA and also on the establishment of a biological signature that relates to specific muscle properties. This hitherto unstudied link between gene/protein signatures and muscle properties may enhance our understanding of axSpA physiopathology and reveal new and useful diagnostic and therapeutic targets.
AB - BACKGROUND: Axial Spondyloarthritis (axSpA) is a chronic, inflammatory rheumatic disease that affects the axial skeleton, causing pain, stiffness, and fatigue. Genetics and environmental factors such as microbiota and microtrauma are known causes of disease susceptibility and progression. Murine models of axSpA found a decisive role for biomechanical stress as an inducer of enthesitis and new bone formation. Here, we hypothesize that muscle properties in axSpA patients are compromised and influenced by genetic background.OBJECTIVES: To improve our current knowledge of axSpA physiopathology, we aim to characterize axial and peripheral muscle properties and identify genetic and protein biomarker that might explain such properties.METHODS: A cross-sectional study will be conducted on 48 participants aged 18-50 years old, involving patients with axSpA (according to ASAS classification criteria, symptoms duration < 10 years) and healthy controls matched by gender, age, and levels of physical activity. We will collect epidemiological and clinical data and perform a detailed, whole body and segmental, myofascial characterization (focusing on multifidus, brachioradialis and the gastrocnemius lateralis) concerning: a) Physical Properties (stiffness, tone and elasticity), assessed by MyotonPRO®; b) Strength, by a dynamometer; c) Mass, by bioimpedance; d) Performance through gait speed and 60-second sit-to-stand test; e) Histological and cellular/ molecular characterization through ultrasound-guided biopsies of multifidus muscle; f) Magnetic Resonance Imaging (MRI) characterization of paravertebral muscles. Furthermore, we will perform an integrated transcriptomics and proteomics analysis of peripheral blood samples.DISCUSSION: The innovative and multidisciplinary approaches of this project rely on the elucidation of myofascial physical properties in axSpA and also on the establishment of a biological signature that relates to specific muscle properties. This hitherto unstudied link between gene/protein signatures and muscle properties may enhance our understanding of axSpA physiopathology and reveal new and useful diagnostic and therapeutic targets.
KW - Adolescent
KW - Adult
KW - Animals
KW - Axial Spondyloarthritis
KW - Cross-Sectional Studies
KW - Humans
KW - Mice
KW - Middle Aged
KW - Muscles
KW - Spondylarthritis
KW - Spondylitis, Ankylosing
KW - Young Adult
UR - https://pubmed.ncbi.nlm.nih.gov/34962249/
M3 - Article
C2 - 34962249
SN - 0303-464X
VL - 46
SP - 342
EP - 349
JO - Acta Reumatológica Portuguesa
JF - Acta Reumatológica Portuguesa
IS - 4
M1 - 1537
ER -
