The relevance of enzyme specificity for coenzymes and the presence of 6-phosphogluconate dehydrogenase for polyhydroxyalkanoates production in the metabolism of Pseudomonas sp. LFM046

Juliana Cardinali Rezende, Alex Di Genova, Rafael A. T. P. S. Nahat, Alexander Steinbüchel, Marie France Sagot, Rafael S. Costa, Henrique C. Oliveira, Marilda K. Taciro, Luiziana F. Silva, José Gregório C. Gomez

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Reconstruction of genome-based metabolic model is a useful approach for the assessment of metabolic pathways, genes and proteins involved in the environmental fitness capabilities or pathogenic potential as well as for biotechnological processes development. Pseudomonas sp. LFM046 was selected as a good polyhydroxyalkanoates (PHA) producer from carbohydrates and plant oils. Its complete genome sequence and metabolic model were obtained. Analysis revealed that the gnd gene, encoding 6-phosphogluconate dehydrogenase, is absent in Pseudomonas sp. LFM046 genome. In order to improve the knowledge about LFM046 metabolism, the coenzyme specificities of different enzymes was evaluated. Furthermore, the heterologous expression of gnd genes from Pseudomonas putida KT2440 (NAD+ dependent) and Escherichia coli MG1655 (NADP+ dependent) in LFM046 was carried out and provoke a delay on cell growth and a reduction in PHA yield, respectively. The results indicate that the adjustment in cyclic Entner-Doudoroff pathway may be an interesting strategy for it and other bacteria to simultaneously meet divergent cell needs during cultivation phases of growth and PHA production.

Original languageEnglish
Pages (from-to)240-250
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume163
DOIs
Publication statusPublished - 15 Nov 2020

Keywords

  • 6-phosphogluconate dehydrogenase
  • Entner-Doudoroff pathway
  • Pentose phosphate pathway
  • PHA production
  • Pseudomonas sp.
  • Reconstruction of genome-based metabolic model

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