The protein family of pyruvate:quinone oxidoreductases: Amino acid sequence conservation and taxonomic distribution

Filipe M. Sousa, Bárbara Fernandes, Manuela M. Pereira

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Abstract

Pyruvate:quinone oxidoreductases (PQOs) catalyse the oxidative decarboxylation of pyruvate to acetate and concomitant reduction of quinone to quinol with the release of CO2. They are thiamine pyrophosphate (TPP) and flavin-adenine dinucleotide (FAD) containing enzymes, which interact with the membrane in a monotopic way. PQOs are considered as part of alternatives to most recognized pyruvate catabolizing pathways, and little is known about their taxonomic distribution and structural/functional relationship. In this bioinformatics work we tackled these gaps in PQO knowledge. We used the KEGG database to identify PQO coding genes, performed a multiple sequence analysis which allowed us to study the amino acid conservation on these enzymes, and looked at their possible cellular function. We observed that PQOS are enzymes exclusively present in prokaryotes with most of the sequences identified in bacteria. Regarding the amino acid sequence conservation, we found that 75 amino acid residues (out of 570, on average) have a conservation over 90 %, and that the most conserved regions in the protein are observed around the TPP and FAD binding sites. We systematized the presence of conserved features involved in Mg2+, TPP and FAD binding, as well as residues directly linked to the catalytic mechanism. We also established the presence of a new motif named “HEH lock”, possibly involved in the dimerization process. The results here obtained for the PQO protein family contribute to a better understanding of the biochemistry of these respiratory enzymes.

Original languageEnglish
Article number148958
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1864
Issue number2
DOIs
Publication statusPublished - 1 Apr 2023

Keywords

  • Amino-acid residue conservation
  • Flavoproteins
  • Monotopic quinone reductases
  • Pyruvate metabolism
  • Respiratory chain
  • Taxonomic profile
  • Thiamine pyrophosphate

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