TY - JOUR
T1 - The primary structure of the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774 reveals an unusual type of diheme cytochrome c
AU - Devreese, Bart
AU - Costa, Cristina
AU - Demol, Hans
AU - Papaefthymiou, Vasilios
AU - Moura, Isabel
AU - Moura, José J. G.
AU - Van Beeumen, Jozef
PY - 1997/1/1
Y1 - 1997/1/1
N2 -
The complete amino acid sequence of the unusual diheme split-Soret cytochrome c from the sulphate-reducing Desulfovibrio, desulfuricans strain ATCC 27774 has been determined using classical chemical sequencing techniques and mass spectrometry. The 247-residue sequence shows almost no similarity with any other known diheme cytochrome c, but the heme-binding site of the protein is similar to that of the cytochromes c
3
from the sulphate reducers. The cytochrome-c-like domain of the protein covers only the C-terminal part of the molecule, and there is evidence for at least one more domain containing four cysteine residues, which might bind another cofactor, possibly a non-heme iron-containing cluster. This domain is similar to a sequence fragment of the genome of Archaeoglobus fulgidus, which confirms the high conservation of the genes involved in sulfate reduction.
AB -
The complete amino acid sequence of the unusual diheme split-Soret cytochrome c from the sulphate-reducing Desulfovibrio, desulfuricans strain ATCC 27774 has been determined using classical chemical sequencing techniques and mass spectrometry. The 247-residue sequence shows almost no similarity with any other known diheme cytochrome c, but the heme-binding site of the protein is similar to that of the cytochromes c
3
from the sulphate reducers. The cytochrome-c-like domain of the protein covers only the C-terminal part of the molecule, and there is evidence for at least one more domain containing four cysteine residues, which might bind another cofactor, possibly a non-heme iron-containing cluster. This domain is similar to a sequence fragment of the genome of Archaeoglobus fulgidus, which confirms the high conservation of the genes involved in sulfate reduction.
KW - Amino acid sequence
KW - Archaeoglobus fulgidus
KW - Desulfovibrio
KW - Mass spectrometry
KW - Sulfate reduction
UR - http://www.scopus.com/inward/record.url?scp=0030886449&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1997.00445.x
DO - 10.1111/j.1432-1033.1997.00445.x
M3 - Article
C2 - 9346301
AN - SCOPUS:0030886449
SN - 0014-2956
VL - 248
SP - 445
EP - 451
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -