The primary structure of the β subunit of Desulfovibrio desulfuricans (ATCC 27774) [NiFe] hydrogenase

Ricardo Franco; Juan J. Calvete; Hubert H. Thole; Manfred Raida; Isabel Moura; José J. G. Moura

The primary structure of the β subunit of Desulfovibrio desulfuricans (ATCC 27774) [NiFe] hydrogenase

Ricardo Franco, Juan J. Calvete, Hubert H. Thole, Manfred Raida, Isabel Moura, José J. G. Moura

Research output: Contribution to journal › Article › peer-review

Abstract

The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (β) and a 60 kDa (α) subunit. Here we report the complete amino acid sequence of the small (β) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.

Original language	English
Pages (from-to)	131-138
Number of pages	8
Journal	Protein And Peptide Letters
Volume	4
Issue number	2
Publication status	Published - Apr 1997

Cite this

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AU - Thole, Hubert H.

AU - Raida, Manfred

AU - Moura, Isabel

AU - Moura, José J. G.

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N2 - The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (β) and a 60 kDa (α) subunit. Here we report the complete amino acid sequence of the small (β) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.

AB - The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (β) and a 60 kDa (α) subunit. Here we report the complete amino acid sequence of the small (β) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.

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ER -

The primary structure of the β subunit of Desulfovibrio desulfuricans (ATCC 27774) [NiFe] hydrogenase

Abstract

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