The primary structure of the β subunit of Desulfovibrio desulfuricans (ATCC 27774) [NiFe] hydrogenase

Ricardo Franco, Juan J. Calvete, Hubert H. Thole, Manfred Raida, Isabel Moura, José J. G. Moura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (β) and a 60 kDa (α) subunit. Here we report the complete amino acid sequence of the small (β) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalProtein And Peptide Letters
Volume4
Issue number2
Publication statusPublished - Apr 1997

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Desulfovibrio desulfuricans
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Protein Sequence Analysis
Mass spectrometry
Amino Acid Sequence
Amino Acids
Degradation
Peptides
Proteins
nickel-iron hydrogenase

Cite this

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abstract = "The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (β) and a 60 kDa (α) subunit. Here we report the complete amino acid sequence of the small (β) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.",
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The primary structure of the β subunit of Desulfovibrio desulfuricans (ATCC 27774) [NiFe] hydrogenase. / Franco, Ricardo; Calvete, Juan J.; Thole, Hubert H.; Raida, Manfred; Moura, Isabel; Moura, José J. G.

In: Protein And Peptide Letters, Vol. 4, No. 2, 04.1997, p. 131-138.

Research output: Contribution to journalArticle

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