The periplasmic [NiFe] hydrogenase isolated from Desulfovibrio (D.) desulfuricans (ATCC 27774) is a heterodimer of a 28 kDa (β) and a 60 kDa (α) subunit. Here we report the complete amino acid sequence of the small (β) polypeptide chain determined by Edman degradation of proteolytic fragments. Electrospray-ionization mass spectrometry of the native protein confirmed the sequencing results. The sequence is compared with that of D. gigas [NiFe] hydrogenase whose three-dimensional structure has been recently published.
|Number of pages||8|
|Journal||Protein And Peptide Letters|
|Publication status||Published - Apr 1997|