The Off-Targets of Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing

Manuel M. Vicente, Miguel Chaves-Ferreira, João M.P. Jorge, João T. Proença, Vasco M. Barreto

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Abstract

The repurposing of the CRISPR/Cas bacterial defense system against bacteriophages as simple and flexible molecular tools has revolutionized the field of gene editing. These tools are now widely used in basic research and clinical trials involving human somatic cells. However, a global moratorium on all clinical uses of human germline editing has been proposed because the technology still lacks the required efficacy and safety. Here we focus on the approaches developed since 2013 to decrease the frequency of unwanted mutations (the off-targets) during CRISPR-based gene editing.

Original languageEnglish
Article number718466
JournalFrontiers in Cell and Developmental Biology
Volume9
DOIs
Publication statusPublished - 17 Sep 2021

Keywords

  • Cas9 variants
  • CRISPR/Cas9
  • DNA repair
  • gene editing
  • off-targets

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