The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus

Patrícia N. Refojo, Filipa Calisto, Miguel A. Ribeiro, Miguel Teixeira, Manuela M. Pereira

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.

Original languageEnglish
Pages (from-to)1037-1044
Number of pages8
JournalBiological Chemistry
Volume398
Issue number9
DOIs
Publication statusPublished - 28 Aug 2017

Fingerprint

Rhodothermus
Electron Transport Complex III
Cytochromes c
Oxidoreductases
Electrons
Hydroquinones
Oxygen
Electron Transport
Lipoproteins

Keywords

  • bc complex
  • cytochrome c oxidase
  • lipoprotein
  • quinone
  • respiratory chain

Cite this

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title = "The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus",
abstract = "Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.",
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TY - JOUR

T1 - The monoheme cytochrome c subunit of Alternative Complex III is a direct electron donor to caa3 oxygen reductase in Rhodothermus marinus

AU - Refojo, Patrícia N.

AU - Calisto, Filipa

AU - Ribeiro, Miguel A.

AU - Teixeira, Miguel

AU - Pereira, Manuela M.

PY - 2017/8/28

Y1 - 2017/8/28

N2 - Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.

AB - Alternative Complex III (ACIII) is an example of the robustness and flexibility of prokaryotic respiratory chains. It performs quinol:cytochrome c oxidoreductase activity, being functionally equivalent to the bc1 complex but structurally unrelated. In this work we further explored ACIII investigating the role of its monoheme cytochrome c subunit (ActE). We expressed and characterized the individually isolated ActE, which allowed us to suggest that ActE is a lipoprotein and to show its function as a direct electron donor to the caa3 oxygen reductase.

KW - bc complex

KW - cytochrome c oxidase

KW - lipoprotein

KW - quinone

KW - respiratory chain

UR - http://www.scopus.com/inward/record.url?scp=85027397121&partnerID=8YFLogxK

U2 - 10.1515/hsz-2016-0323

DO - 10.1515/hsz-2016-0323

M3 - Article

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SP - 1037

EP - 1044

JO - Biological Chemistry

JF - Biological Chemistry

SN - 1431-6730

IS - 9

ER -