The link between nitrous oxide emissions, microbial community profile and function from three full-scale WWTPs

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Abstract

Few attempts have been made in previous studies to link the microbial community structure and function with nitrous oxide (N2O) emissions at full-scale wastewater treatment plants (WWTPs). In this work, high-throughput sequencing and reverse transcriptase-qPCR (RT-qPCR) was applied to activated sludge samples from three WWTPs for two seasonal periods (winter and summer) and linked with the N2O emissions and wastewater characteristics. The total N2O emissions ranged from 7.2 to 937.0 g N-N2O/day, which corresponds to an emission factor of 0.001 to 0.280% of the influent NH4-N being emitted as N2O. Those emissions were related to the abundance of Nitrotoga, Candidatus Microthrix and Rhodobacter genera, which were favored by higher dissolved oxygen (DO) and nitrate (NO3 ) concentrations in the activated sludge tanks. Furthermore, a relationship between the nirK gene expression and N2O emissions was verified. Detected N2O emission peaks were associated with different process events, related to aeration transition periods, that occurred during the regular operation of the plants, which could be potentially associated to increased emissions of the WWTP. The design of mitigation strategies, such as optimizing the aeration regime, is therefore important to avoid process events that lead to those N2O emissions peaks. Furthermore, this study also demonstrates the importance of assessing the gene expression of nosZ clade II, since its high abundance in WWTPs could be an important key to reduce the N2O emissions.

LanguageEnglish
Pages2460-2472
Number of pages13
JournalScience of the Total Environment
Volume651
DOIs
Publication statusPublished - 15 Feb 2019

Fingerprint

Nitrous Oxide
nitrous oxide
Wastewater treatment
microbial community
Oxides
Gene expression
RNA-Directed DNA Polymerase
Dissolved oxygen
Nitrates
aeration
Wastewater
gene expression
activated sludge
Throughput
wastewater treatment plant
dissolved oxygen
community structure
mitigation
nitrate
wastewater

Keywords

  • Denitrification
  • Greenhouse gas (GHG)
  • Nitrification
  • Nitrous oxide (NO)
  • Nitrous oxide reductase (NosZ)
  • Wastewater treatment plants (WWTPs)

Cite this

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title = "The link between nitrous oxide emissions, microbial community profile and function from three full-scale WWTPs",
abstract = "Few attempts have been made in previous studies to link the microbial community structure and function with nitrous oxide (N2O) emissions at full-scale wastewater treatment plants (WWTPs). In this work, high-throughput sequencing and reverse transcriptase-qPCR (RT-qPCR) was applied to activated sludge samples from three WWTPs for two seasonal periods (winter and summer) and linked with the N2O emissions and wastewater characteristics. The total N2O emissions ranged from 7.2 to 937.0 g N-N2O/day, which corresponds to an emission factor of 0.001 to 0.280{\%} of the influent NH4-N being emitted as N2O. Those emissions were related to the abundance of Nitrotoga, Candidatus Microthrix and Rhodobacter genera, which were favored by higher dissolved oxygen (DO) and nitrate (NO3 −) concentrations in the activated sludge tanks. Furthermore, a relationship between the nirK gene expression and N2O emissions was verified. Detected N2O emission peaks were associated with different process events, related to aeration transition periods, that occurred during the regular operation of the plants, which could be potentially associated to increased emissions of the WWTP. The design of mitigation strategies, such as optimizing the aeration regime, is therefore important to avoid process events that lead to those N2O emissions peaks. Furthermore, this study also demonstrates the importance of assessing the gene expression of nosZ clade II, since its high abundance in WWTPs could be an important key to reduce the N2O emissions.",
keywords = "Denitrification, Greenhouse gas (GHG), Nitrification, Nitrous oxide (NO), Nitrous oxide reductase (NosZ), Wastewater treatment plants (WWTPs)",
author = "A. Vieira and Galinha, {C. F.} and A. Oehmen and G. Carvalho",
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TY - JOUR

T1 - The link between nitrous oxide emissions, microbial community profile and function from three full-scale WWTPs

AU - Vieira, A.

AU - Galinha, C. F.

AU - Oehmen, A.

AU - Carvalho, G.

N1 - Sem Pdf conforme despacho. Fundacao para a Ciencia e Tecnologia (FCT/MCES) -PTDC/AAC-AMB/120581/2010 # European Commission # Portuguese Research Unit UCIBIO - FCT/MCES - UID/Multi/04378/2013 # Portuguese Research Unit iNOVA4Health - FCT/MCES -UID/Multi/04378/2013 # FEDER under the PT2020 Partnership Agreement -POCI-01-0145-FEDER-007728 LISBOA-01-0145-FEDER-007344# FCT/MCES-SFRH/BD/97640/2013 SFRH/BPD/95864/2013.

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Few attempts have been made in previous studies to link the microbial community structure and function with nitrous oxide (N2O) emissions at full-scale wastewater treatment plants (WWTPs). In this work, high-throughput sequencing and reverse transcriptase-qPCR (RT-qPCR) was applied to activated sludge samples from three WWTPs for two seasonal periods (winter and summer) and linked with the N2O emissions and wastewater characteristics. The total N2O emissions ranged from 7.2 to 937.0 g N-N2O/day, which corresponds to an emission factor of 0.001 to 0.280% of the influent NH4-N being emitted as N2O. Those emissions were related to the abundance of Nitrotoga, Candidatus Microthrix and Rhodobacter genera, which were favored by higher dissolved oxygen (DO) and nitrate (NO3 −) concentrations in the activated sludge tanks. Furthermore, a relationship between the nirK gene expression and N2O emissions was verified. Detected N2O emission peaks were associated with different process events, related to aeration transition periods, that occurred during the regular operation of the plants, which could be potentially associated to increased emissions of the WWTP. The design of mitigation strategies, such as optimizing the aeration regime, is therefore important to avoid process events that lead to those N2O emissions peaks. Furthermore, this study also demonstrates the importance of assessing the gene expression of nosZ clade II, since its high abundance in WWTPs could be an important key to reduce the N2O emissions.

AB - Few attempts have been made in previous studies to link the microbial community structure and function with nitrous oxide (N2O) emissions at full-scale wastewater treatment plants (WWTPs). In this work, high-throughput sequencing and reverse transcriptase-qPCR (RT-qPCR) was applied to activated sludge samples from three WWTPs for two seasonal periods (winter and summer) and linked with the N2O emissions and wastewater characteristics. The total N2O emissions ranged from 7.2 to 937.0 g N-N2O/day, which corresponds to an emission factor of 0.001 to 0.280% of the influent NH4-N being emitted as N2O. Those emissions were related to the abundance of Nitrotoga, Candidatus Microthrix and Rhodobacter genera, which were favored by higher dissolved oxygen (DO) and nitrate (NO3 −) concentrations in the activated sludge tanks. Furthermore, a relationship between the nirK gene expression and N2O emissions was verified. Detected N2O emission peaks were associated with different process events, related to aeration transition periods, that occurred during the regular operation of the plants, which could be potentially associated to increased emissions of the WWTP. The design of mitigation strategies, such as optimizing the aeration regime, is therefore important to avoid process events that lead to those N2O emissions peaks. Furthermore, this study also demonstrates the importance of assessing the gene expression of nosZ clade II, since its high abundance in WWTPs could be an important key to reduce the N2O emissions.

KW - Denitrification

KW - Greenhouse gas (GHG)

KW - Nitrification

KW - Nitrous oxide (NO)

KW - Nitrous oxide reductase (NosZ)

KW - Wastewater treatment plants (WWTPs)

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U2 - 10.1016/j.scitotenv.2018.10.132

DO - 10.1016/j.scitotenv.2018.10.132

M3 - Article

VL - 651

SP - 2460

EP - 2472

JO - The Science of the total environment

T2 - The Science of the total environment

JF - The Science of the total environment

SN - 0048-9697

ER -