TY - JOUR
T1 - The in vitro activity of products formed from exposure of chlorpromazine to a 266nm laser beam against species of mycobacteria of human interest
AU - Armada, Ana Maria
AU - Alexandru, Tatiana
AU - Machado, Diana
AU - Danko, Balazs
AU - Hunyadi, Attila
AU - Dinache, Andra
AU - Nastasa, Viorel
AU - Boni, Mihai
AU - Ramos, Jorge
AU - Viveiros, Miguel
AU - Molnar, Joseph
AU - Pascu, Mihail Lucian
AU - Amaral, Leonard
PY - 2013/9
Y1 - 2013/9
N2 - Chlorpromazine (CPZ) was exposed to a 266nm laser beam for different periods of time ranging from minutes to 24 h. At intervals, the products from irradiation were evaluated by thin-layer chromatography (TLC) and evaluated for their activity against mycobacteria of human interest (Mycobacterium tuberculosis, M. avium, M. intracellulare and their corresponding reference strains or clinical isolates). With the exception of the M. avium 47/07 clinical strain, the products produced from the irradiation of CPZ for 4 h had greater activity against M. intracellulare ATCC, M. avium ATCC, H37Rv and the Multidrug-resistant tuberculosis (MDRTB) strains as opposed to that produced by the unirradiated control. The level of products from the 4-h exposure of CPZ remained the same throughout the next 20 h of irradiation. Of significant note is that the irradiation products of CPZ had lower in vitro cytotoxicity against human cells, suggesting that this approach may be useful for the development of compounds more bioactive than the parental species.
AB - Chlorpromazine (CPZ) was exposed to a 266nm laser beam for different periods of time ranging from minutes to 24 h. At intervals, the products from irradiation were evaluated by thin-layer chromatography (TLC) and evaluated for their activity against mycobacteria of human interest (Mycobacterium tuberculosis, M. avium, M. intracellulare and their corresponding reference strains or clinical isolates). With the exception of the M. avium 47/07 clinical strain, the products produced from the irradiation of CPZ for 4 h had greater activity against M. intracellulare ATCC, M. avium ATCC, H37Rv and the Multidrug-resistant tuberculosis (MDRTB) strains as opposed to that produced by the unirradiated control. The level of products from the 4-h exposure of CPZ remained the same throughout the next 20 h of irradiation. Of significant note is that the irradiation products of CPZ had lower in vitro cytotoxicity against human cells, suggesting that this approach may be useful for the development of compounds more bioactive than the parental species.
KW - 266nm
KW - Chlorpromazine
KW - Cytotoxicity of products
KW - Laser beam
KW - Mycobacteria
UR - http://www.scopus.com/inward/record.url?scp=84885643473&partnerID=8YFLogxK
UR - http://iv.iiarjournals.org/content/27/5/605.long
M3 - Article
C2 - 23988894
AN - SCOPUS:84885643473
VL - 27
SP - 605
EP - 610
JO - In vivo (Athens, Greece)
JF - In vivo (Athens, Greece)
SN - 1791-7549
IS - 5
ER -