TY - JOUR
T1 - The Impact of the Virulence of Pseudomonas aeruginosa Isolated from Dogs
AU - de Sousa, Telma
AU - Garcês, Andreia
AU - Silva, Augusto
AU - Lopes, Ricardo
AU - Alegria, Nuno
AU - Hébraud, Michel
AU - Igrejas, Gilberto
AU - Poeta, Patricia
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT#
Funding Information:
This research was funded by FCT (Fundação para a Ciência e a Tecnologia) related to PhD grant, through the reference DFA/BD/5332/2020.
Publisher Copyright:
© 2023 by the authors.
PY - 2023/5/11
Y1 - 2023/5/11
N2 - Pseudomonas aeruginosa is a pathogenic bacterium that can cause serious infections in both humans and animals, including dogs. Treatment of this bacterium is challenging because some strains have developed multi-drug resistance. This study aimed to evaluate the antimicrobial resistance patterns and biofilm production of clinical isolates of P. aeruginosa obtained from dogs. The study found that resistance to various β-lactam antimicrobials was widespread, with cefovecin and ceftiofur showing resistance in 74% and 59% of the isolates tested, respectively. Among the aminoglycosides, all strains showed susceptibility to amikacin and tobramycin, while gentamicin resistance was observed in 7% of the tested isolates. Furthermore, all isolates carried the oprD gene, which is essential in governing the entry of antibiotics into bacterial cells. The study also investigated the presence of virulence genes and found that all isolates carried exoS, exoA, exoT, exoY, aprA, algD, and plcH genes. This study compared P. aeruginosa resistance patterns worldwide, emphasizing regional understanding and responsible antibiotic use to prevent multi-drug resistance from emerging. In general, the results of this study emphasize the importance of the continued monitoring of antimicrobial resistance in veterinary medicine.
AB - Pseudomonas aeruginosa is a pathogenic bacterium that can cause serious infections in both humans and animals, including dogs. Treatment of this bacterium is challenging because some strains have developed multi-drug resistance. This study aimed to evaluate the antimicrobial resistance patterns and biofilm production of clinical isolates of P. aeruginosa obtained from dogs. The study found that resistance to various β-lactam antimicrobials was widespread, with cefovecin and ceftiofur showing resistance in 74% and 59% of the isolates tested, respectively. Among the aminoglycosides, all strains showed susceptibility to amikacin and tobramycin, while gentamicin resistance was observed in 7% of the tested isolates. Furthermore, all isolates carried the oprD gene, which is essential in governing the entry of antibiotics into bacterial cells. The study also investigated the presence of virulence genes and found that all isolates carried exoS, exoA, exoT, exoY, aprA, algD, and plcH genes. This study compared P. aeruginosa resistance patterns worldwide, emphasizing regional understanding and responsible antibiotic use to prevent multi-drug resistance from emerging. In general, the results of this study emphasize the importance of the continued monitoring of antimicrobial resistance in veterinary medicine.
KW - antibiotic resistance
KW - dogs
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85160215463&partnerID=8YFLogxK
U2 - 10.3390/vetsci10050343
DO - 10.3390/vetsci10050343
M3 - Article
C2 - 37235426
AN - SCOPUS:85160215463
SN - 2306-7381
VL - 10
JO - Veterinary Sciences
JF - Veterinary Sciences
IS - 5
M1 - 343
ER -