TY - JOUR
T1 - The impact of pH on the anaerobic and aerobic metabolism of Tetrasphaera-enriched polyphosphate accumulating organisms
AU - Nguyen, P. Y.
AU - Marques, Ricardo
AU - Wang, Hongmin
AU - Reis, Maria A. M.
AU - Carvalho, Gilda
AU - Oehmen, Adrian
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT#
Funding Information:
The authors thank the Portuguese Fundaçao para a Ciência e a Tecnologia, which supports the Applied Molecular Biosciences Unit - UCIBIO, the European Commission (Water JPI project 196 (Water-Works2014 ERA-NET Co-funded Call): “Smart decentralized water management through a dynamic integration of technologies (Watintech)” and the Australian Research Council ( ARC LP190100329 ).
Publisher Copyright:
© 2023
PY - 2023/5/1
Y1 - 2023/5/1
N2 - Members of the genus Tetrasphaera are putative polyphosphate accumulating organisms (PAOs) that have been found in greater abundance than Accumulibacter in many full-scale enhanced biological phosphorus removal (EBPR) wastewater treatment plants worldwide. Nevertheless, previous studies on the effect of environmental conditions, such as pH, on the performance of EBPR have focused mainly on the response of Accumulibacter to pH changes. This study examines the impact of pH on a Tetrasphaera PAO enriched culture, over a pH range from 6.0 to 8.0 under both anaerobic and aerobic conditions, to assess its impact on the stoichiometry and kinetics of Tetrasphaera metabolism. It was discovered that the rates of phosphorus (P) uptake and P release increased with an increase of pH within the tested range, while PHA production, glycogen consumption and substrate uptake rate were less sensitive to pH changes. The results suggest that Tetrasphaera PAOs display kinetic advantages at high pH levels, which is consistent with what has been observed previously for Accumulibacter PAOs. The results of this study show that pH has a substantial impact on the P release and uptake kinetics of PAOs, where the P release rate was >3 times higher and the P uptake rate was >2 times higher at pH 8.0 vs pH 6.0, respectively. Process operational strategies promoting both Tetrasphaera and Accumulibacter activity at high pH do not conflict with each other, but lead to a potentially synergistic impact that can benefit EBPR performance.
AB - Members of the genus Tetrasphaera are putative polyphosphate accumulating organisms (PAOs) that have been found in greater abundance than Accumulibacter in many full-scale enhanced biological phosphorus removal (EBPR) wastewater treatment plants worldwide. Nevertheless, previous studies on the effect of environmental conditions, such as pH, on the performance of EBPR have focused mainly on the response of Accumulibacter to pH changes. This study examines the impact of pH on a Tetrasphaera PAO enriched culture, over a pH range from 6.0 to 8.0 under both anaerobic and aerobic conditions, to assess its impact on the stoichiometry and kinetics of Tetrasphaera metabolism. It was discovered that the rates of phosphorus (P) uptake and P release increased with an increase of pH within the tested range, while PHA production, glycogen consumption and substrate uptake rate were less sensitive to pH changes. The results suggest that Tetrasphaera PAOs display kinetic advantages at high pH levels, which is consistent with what has been observed previously for Accumulibacter PAOs. The results of this study show that pH has a substantial impact on the P release and uptake kinetics of PAOs, where the P release rate was >3 times higher and the P uptake rate was >2 times higher at pH 8.0 vs pH 6.0, respectively. Process operational strategies promoting both Tetrasphaera and Accumulibacter activity at high pH do not conflict with each other, but lead to a potentially synergistic impact that can benefit EBPR performance.
KW - Accumulibacter
KW - amino acids
KW - Enhanced Biological Phosphorus Removal (EBPR)
KW - polyhydroxyalkanoate (PHA)
KW - Polyphosphate Accumulating Organisms (PAOs)
KW - Tetrasphaera
UR - http://www.scopus.com/inward/record.url?scp=85151246901&partnerID=8YFLogxK
U2 - 10.1016/j.wroa.2023.100177
DO - 10.1016/j.wroa.2023.100177
M3 - Article
C2 - 37008369
AN - SCOPUS:85151246901
SN - 2589-9147
VL - 19
JO - Water Research X
JF - Water Research X
M1 - 100177
ER -