The host endocytic pathway is essential for plasmodium berghei late liver stage development

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28 Citations (Scopus)

Abstract

The obligate intracellular liver stage of the Plasmodium parasite represents a bottleneck in the parasite life cycle and remains a promising target for therapeutic intervention. During this stage, parasites undergo dramatic morphological changes and achieve one of the fastest replication rates among eukaryotic species. Nevertheless, relatively little is known about the parasite interactions with the host hepatocyte. Using immunofluorescence, live cell imaging and electron microscopy, we show that Plasmodium berghei parasites are surrounded by vesicles from the host late endocytic pathway. We found that these vesicles are acidic and contain the membrane markers Rab7a, CD63 and LAMP1. When host cell vesicle acidification was disrupted using ammonium chloride or Concanamycin A during the late liver stage of infection, parasite survival was not affected, but schizont size was significantly decreased. Furthermore, when the host cell endocytic pathway was loaded with BSA-gold, gold particles were found within the parasite cytoplasm, showing the transport of material from the host endocytic pathway toward the parasite interior. These observations reveal a novel Plasmodium-host interaction and suggest that vesicles from the host endolysosomal pathway could represent an important source of nutrients exploited by the fast-growing late liver stage parasites.

Original languageEnglish
Pages (from-to)1351-1363
Number of pages13
JournalTraffic
Volume13
Issue number10
DOIs
Publication statusPublished - 1 Oct 2012

Fingerprint

Plasmodium berghei
Liver
Parasites
Plasmodium
Gold
Schizonts
Host-Parasite Interactions
Ammonium Chloride
Parasitic Diseases
Life Cycle Stages
Acidification
Fluorescent Antibody Technique
Hepatocytes
Electron Microscopy
Cytoplasm
Electron microscopy
Nutrients
Life cycle
Food
Membranes

Keywords

  • Endocytic pathway
  • Late endosome
  • Liver stage infection
  • Lysosome
  • Malaria
  • Plasmodium berghei

Cite this

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title = "The host endocytic pathway is essential for plasmodium berghei late liver stage development",
abstract = "The obligate intracellular liver stage of the Plasmodium parasite represents a bottleneck in the parasite life cycle and remains a promising target for therapeutic intervention. During this stage, parasites undergo dramatic morphological changes and achieve one of the fastest replication rates among eukaryotic species. Nevertheless, relatively little is known about the parasite interactions with the host hepatocyte. Using immunofluorescence, live cell imaging and electron microscopy, we show that Plasmodium berghei parasites are surrounded by vesicles from the host late endocytic pathway. We found that these vesicles are acidic and contain the membrane markers Rab7a, CD63 and LAMP1. When host cell vesicle acidification was disrupted using ammonium chloride or Concanamycin A during the late liver stage of infection, parasite survival was not affected, but schizont size was significantly decreased. Furthermore, when the host cell endocytic pathway was loaded with BSA-gold, gold particles were found within the parasite cytoplasm, showing the transport of material from the host endocytic pathway toward the parasite interior. These observations reveal a novel Plasmodium-host interaction and suggest that vesicles from the host endolysosomal pathway could represent an important source of nutrients exploited by the fast-growing late liver stage parasites.",
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author = "{Lopes da Silva}, Mafalda and Carolina Thieleke-Matos and Laura Cabrita-Santos and Ramalho, {Jos{\'e} S.} and Wavre-Shapton, {Sil{\`e}ne T.} and Futter, {Clare E.} and Barral, {Duarte C.} and Seabra, {Miguel C.}",
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AU - Lopes da Silva, Mafalda

AU - Thieleke-Matos, Carolina

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AU - Ramalho, José S.

AU - Wavre-Shapton, Silène T.

AU - Futter, Clare E.

AU - Barral, Duarte C.

AU - Seabra, Miguel C.

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