The homopentameric chlorite dismutase from Magnetospirillum sp.

Diana M. Freire, Maria G. Rivas, André M. Dias, Ana T. Lopes, Cristina Costa, Teresa Santos-Silva, Sabine Van Doorslaer, Pablo J. González

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Chlorite dismutase (Cld) is a b-type heme containing enzyme that catalyzes the reduction of chlorite into chloride plus dioxygen. This enzyme has gained attention because it can be used in the development of bioremediation processes, biosensors, and controlled dioxygen production. In the present work, Cld was purified from Magnetospirillum sp. cells cultured anaerobically with acetate/perchlorate until stationary phase. Biochemical, spectroscopic and X-ray crystallography methods showed that Cld from Magnetospirillum sp. is a ~ 140 kDa homopentamer comprising ~ 27.8 kDa monomers. Preliminary X-ray crystallography studies confirmed the quaternary structure and the presence of one b-type heme per monomer. The EPR spectroscopic signature of the as-purified Cld samples is affected by the buffer composition used during the purification. Potassium phosphate buffer is the only buffer that affected neither the spectral nor the kinetic properties of Cld. Kinetic studies in solution revealed that Cld from Magnetospirillum sp. decomposes chlorite at high turnover rates with optimal pH 6.0. A temperature below 10 °C is required to avoid enzyme inactivation due to cofactor bleaching during turnover, and to achieve full substrate consumption. Cld kinetic parameters were not affected when kinetic assays were performed in the presence of air or under argon atmosphere, but chloride is a weak mixed inhibitor that modifies the EPR signal of as-prepared samples.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume151
DOIs
Publication statusPublished - 27 Jul 2015

Keywords

  • Chlorite dismutase
  • Enzyme kinetics
  • EPR spectroscopy
  • Magnetospirillum
  • Perchlorate-reducing bacteria
  • X-ray crystallography

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