TY - JOUR
T1 - The Henna pigment Lawsone activates the Aryl Hydrocarbon Receptor and impacts skin homeostasis
AU - Lozza, Laura
AU - Moura-Alves, Pedro
AU - Domaszewska, Teresa
AU - Lage Crespo, Carolina
AU - Streata, Ioana
AU - Kreuchwig, Annika
AU - Puyskens, Andreas
AU - Bechtle, Marina
AU - Klemm, Marion
AU - Zedler, Ulrike
AU - Silviu Ungureanu, Bogdan
AU - Guhlich-Bornhof, Ute
AU - Koehler, Anne Britta
AU - Stäber, Manuela
AU - Mollenkopf, Hans Joachim
AU - Hurwitz, Robert
AU - Furkert, Jens
AU - Krause, Gerd
AU - Weiner, January
AU - Jacinto, António
AU - Mihai, Ioana
AU - Leite-de-Moraes, Maria
AU - Siebenhaar, Frank
AU - Maurer, Marcus
AU - Kaufmann, Stefan H.E.
PY - 2019/7/26
Y1 - 2019/7/26
N2 - As a first host barrier, the skin is constantly exposed to environmental insults that perturb its integrity. Tight regulation of skin homeostasis is largely controlled by the aryl hydrocarbon receptor (AhR). Here, we demonstrate that Henna and its major pigment, the naphthoquinone Lawsone activate AhR, both in vitro and in vivo. In human keratinocytes and epidermis equivalents, Lawsone exposure enhances the production of late epidermal proteins, impacts keratinocyte differentiation and proliferation, and regulates skin inflammation. To determine the potential use of Lawsone for therapeutic application, we harnessed human, murine and zebrafish models. In skin regeneration models, Lawsone interferes with physiological tissue regeneration and inhibits wound healing. Conversely, in a human acute dermatitis model, topical application of a Lawsone-containing cream ameliorates skin irritation. Altogether, our study reveals how a widely used natural plant pigment is sensed by the host receptor AhR, and how the physiopathological context determines beneficial and detrimental outcomes.
AB - As a first host barrier, the skin is constantly exposed to environmental insults that perturb its integrity. Tight regulation of skin homeostasis is largely controlled by the aryl hydrocarbon receptor (AhR). Here, we demonstrate that Henna and its major pigment, the naphthoquinone Lawsone activate AhR, both in vitro and in vivo. In human keratinocytes and epidermis equivalents, Lawsone exposure enhances the production of late epidermal proteins, impacts keratinocyte differentiation and proliferation, and regulates skin inflammation. To determine the potential use of Lawsone for therapeutic application, we harnessed human, murine and zebrafish models. In skin regeneration models, Lawsone interferes with physiological tissue regeneration and inhibits wound healing. Conversely, in a human acute dermatitis model, topical application of a Lawsone-containing cream ameliorates skin irritation. Altogether, our study reveals how a widely used natural plant pigment is sensed by the host receptor AhR, and how the physiopathological context determines beneficial and detrimental outcomes.
KW - KERATINOCYTES
KW - EXPRESSION
KW - DIOXIN
KW - AHR
KW - DIFFERENTIATION
KW - 2,3,7,8
KW - TETRACHLORODIBENZO-PARA-DIOXIN
KW - IDENTIFICATION
KW - DERMATITIS
KW - INDUCTION
KW - TOXICITY
UR - http://www.scopus.com/inward/record.url?scp=85069718136&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-47350-x
DO - 10.1038/s41598-019-47350-x
M3 - Article
C2 - 31350436
AN - SCOPUS:85069718136
VL - 9
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 10878
ER -