The harmonized INFOGEST in vitro digestion method

From knowledge to action

Lotti Egger, Olivia Ménard, Cristina Delgado-Andrade, Paula Alvito, Ricardo Assunção, Simon Balance, Reyes Barberá, Andre Brodkorb, Thomas Cattenoz, Alfonso Clemente, Irene Comi, Didier Dupont, Guadalupe Garcia-Llatas, María Jesús Lagarda, Steven Le Feunteun, Lonneke JanssenDuijghuijsen, Sibel Karakaya, Uri Lesmes, Alan R. Mackie, Carla Martins & 18 others Anne Meynier, Beatriz Miralles, Brent S. Murray, Anne Pihlanto, Gianluca Picariello, Claudia N. Santos, Sebnem Simsek, Isidra Recio, Neil Rigby, Laurie Eve Rioux, Helena Stoffers, Ana Tavares, Lucelia Tavares, Sylvie Turgeon, Ellen K. Ulleberg, Gerd E. Vegarud, Guy Vergères, Reto Portmann

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas β-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The present work gives an overview of all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalFood Research International
Volume88
DOIs
Publication statusPublished - 1 Oct 2016

Fingerprint

in vitro digestion
Digestion
pepsin
Pepsin A
stomach
lactoglobulins
digestion
Stomach
methodology
food research
dried skim milk
protein hydrolysates
Food
Lactoglobulins
free amino acids
Caseins
casein
Powders
hydrolysis
In Vitro Techniques

Keywords

  • Dairy proteins
  • Harmonized IVD protocol
  • In vitro digestion
  • Inter-laboratory trial
  • Mass spectrometry
  • Peptides

Cite this

Egger, L., Ménard, O., Delgado-Andrade, C., Alvito, P., Assunção, R., Balance, S., ... Portmann, R. (2016). The harmonized INFOGEST in vitro digestion method: From knowledge to action. Food Research International, 88, 217-225. https://doi.org/10.1016/j.foodres.2015.12.006
Egger, Lotti ; Ménard, Olivia ; Delgado-Andrade, Cristina ; Alvito, Paula ; Assunção, Ricardo ; Balance, Simon ; Barberá, Reyes ; Brodkorb, Andre ; Cattenoz, Thomas ; Clemente, Alfonso ; Comi, Irene ; Dupont, Didier ; Garcia-Llatas, Guadalupe ; Lagarda, María Jesús ; Le Feunteun, Steven ; JanssenDuijghuijsen, Lonneke ; Karakaya, Sibel ; Lesmes, Uri ; Mackie, Alan R. ; Martins, Carla ; Meynier, Anne ; Miralles, Beatriz ; Murray, Brent S. ; Pihlanto, Anne ; Picariello, Gianluca ; Santos, Claudia N. ; Simsek, Sebnem ; Recio, Isidra ; Rigby, Neil ; Rioux, Laurie Eve ; Stoffers, Helena ; Tavares, Ana ; Tavares, Lucelia ; Turgeon, Sylvie ; Ulleberg, Ellen K. ; Vegarud, Gerd E. ; Vergères, Guy ; Portmann, Reto. / The harmonized INFOGEST in vitro digestion method : From knowledge to action. In: Food Research International. 2016 ; Vol. 88. pp. 217-225.
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Egger, L, Ménard, O, Delgado-Andrade, C, Alvito, P, Assunção, R, Balance, S, Barberá, R, Brodkorb, A, Cattenoz, T, Clemente, A, Comi, I, Dupont, D, Garcia-Llatas, G, Lagarda, MJ, Le Feunteun, S, JanssenDuijghuijsen, L, Karakaya, S, Lesmes, U, Mackie, AR, Martins, C, Meynier, A, Miralles, B, Murray, BS, Pihlanto, A, Picariello, G, Santos, CN, Simsek, S, Recio, I, Rigby, N, Rioux, LE, Stoffers, H, Tavares, A, Tavares, L, Turgeon, S, Ulleberg, EK, Vegarud, GE, Vergères, G & Portmann, R 2016, 'The harmonized INFOGEST in vitro digestion method: From knowledge to action', Food Research International, vol. 88, pp. 217-225. https://doi.org/10.1016/j.foodres.2015.12.006

The harmonized INFOGEST in vitro digestion method : From knowledge to action. / Egger, Lotti; Ménard, Olivia; Delgado-Andrade, Cristina; Alvito, Paula; Assunção, Ricardo; Balance, Simon; Barberá, Reyes; Brodkorb, Andre; Cattenoz, Thomas; Clemente, Alfonso; Comi, Irene; Dupont, Didier; Garcia-Llatas, Guadalupe; Lagarda, María Jesús; Le Feunteun, Steven; JanssenDuijghuijsen, Lonneke; Karakaya, Sibel; Lesmes, Uri; Mackie, Alan R.; Martins, Carla; Meynier, Anne; Miralles, Beatriz; Murray, Brent S.; Pihlanto, Anne; Picariello, Gianluca; Santos, Claudia N.; Simsek, Sebnem; Recio, Isidra; Rigby, Neil; Rioux, Laurie Eve; Stoffers, Helena; Tavares, Ana; Tavares, Lucelia; Turgeon, Sylvie; Ulleberg, Ellen K.; Vegarud, Gerd E.; Vergères, Guy; Portmann, Reto.

In: Food Research International, Vol. 88, 01.10.2016, p. 217-225.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The harmonized INFOGEST in vitro digestion method

T2 - From knowledge to action

AU - Egger, Lotti

AU - Ménard, Olivia

AU - Delgado-Andrade, Cristina

AU - Alvito, Paula

AU - Assunção, Ricardo

AU - Balance, Simon

AU - Barberá, Reyes

AU - Brodkorb, Andre

AU - Cattenoz, Thomas

AU - Clemente, Alfonso

AU - Comi, Irene

AU - Dupont, Didier

AU - Garcia-Llatas, Guadalupe

AU - Lagarda, María Jesús

AU - Le Feunteun, Steven

AU - JanssenDuijghuijsen, Lonneke

AU - Karakaya, Sibel

AU - Lesmes, Uri

AU - Mackie, Alan R.

AU - Martins, Carla

AU - Meynier, Anne

AU - Miralles, Beatriz

AU - Murray, Brent S.

AU - Pihlanto, Anne

AU - Picariello, Gianluca

AU - Santos, Claudia N.

AU - Simsek, Sebnem

AU - Recio, Isidra

AU - Rigby, Neil

AU - Rioux, Laurie Eve

AU - Stoffers, Helena

AU - Tavares, Ana

AU - Tavares, Lucelia

AU - Turgeon, Sylvie

AU - Ulleberg, Ellen K.

AU - Vegarud, Gerd E.

AU - Vergères, Guy

AU - Portmann, Reto

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Within the active field of in vitro digestion in food research, the COST Action INFOGEST aimed to harmonize in vitro protocols simulating human digestion on the basis of physiologically inferred conditions. A harmonized static in vitro digestion (IVD) method was recently published as a primary output from this network. To validate this protocol, inter-laboratory trials were conducted within the INFOGEST network. A first study was performed using skim milk powder (SMP) as a model food and served to compare the different in-house digestion protocols used among the INFOGEST members. In a second inter-laboratory study applying the harmonized protocol, the degree of consistency in protein hydrolysis was investigated. Analysis of the hydrolyzed proteins, after the gastric and intestinal phases, showed that caseins were mainly hydrolyzed during the gastric phase, whereas β-lactoglobulin was, as previously shown, resistant to pepsin. Moreover, generation of free amino acids occurred mainly during the intestinal phase. The study also showed that a few critical steps were responsible for the remaining inter-laboratory variability. The largest deviations arose from the determination of pepsin activity. Therefore, this step was further clarified, harmonized, and implemented in a third inter-laboratory study. The present work gives an overview of all three inter-laboratory studies, showing that the IVD INFOGEST method has led to an increased consistency that enables a better comparability of in vitro digestion studies in the future.

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KW - Dairy proteins

KW - Harmonized IVD protocol

KW - In vitro digestion

KW - Inter-laboratory trial

KW - Mass spectrometry

KW - Peptides

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Egger L, Ménard O, Delgado-Andrade C, Alvito P, Assunção R, Balance S et al. The harmonized INFOGEST in vitro digestion method: From knowledge to action. Food Research International. 2016 Oct 1;88:217-225. https://doi.org/10.1016/j.foodres.2015.12.006