The glucosaminidase domain of Atl - the major Staphylococcus aureus autolysin - has DNA-binding activity

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13 Citations (Scopus)

Abstract

In this communication, we describe evidence demonstrating the capacity of Atl, the major Staphylococcus aureus autolytic enzyme to bind DNA. Electrophoretic mobility shift assays (EMSA) show that both the Atl protein and the endo-β-N-acetylglucosaminidase (GL) domain were able to bind DNA of nonspecific sequence. The implications of this unexpected observation for the physiology of S. aureus remain to be explored. In this communication, we describe the capacity of Atl, the major Staphylococcus aureus autolytic enzyme, to bind DNA. Both the Atl protein and the endo-β-N-acetylglucosaminidase (GL) domain were able to bind DNA of nonspecific sequence. The implications of this unexpected observation for the physiology of Staphylococcus aureus remain to be explored.

Original languageEnglish
Pages (from-to)247-256
Number of pages10
JournalMicrobiologyopen
Volume3
Issue number2
DOIs
Publication statusPublished - Apr 2014

Keywords

  • Atl autolysin
  • DNA binding
  • Protein-DNA interaction
  • Staphylococcus aureus

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