TY - JOUR
T1 - The Biogenic Synthesis of Bimetallic Ag/ZnO Nanoparticles
T2 - A Multifunctional Approach for Methyl Violet Photocatalytic Degradation and the Assessment of Antibacterial, Antioxidant, and Cytotoxicity Properties
AU - Afzal, Muhammad Asjad
AU - Javed, Muhammad
AU - Aroob, Sadia
AU - Javed, Tariq
AU - M. Alnoman, Maryam
AU - Alelwani, Walla
AU - Bibi, Ismat
AU - Sharif, Muhammad
AU - Saleem, Muhammad
AU - Rizwan, Muhammad
AU - Raheel, Ahmad
AU - Maseeh, Ihsan
AU - Carabineiro, Sónia A. C.
AU - Taj, Muhammad Babar
N1 - info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT#
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT#
Funding Information:
M.B.T acknowledges support from HEC (Higher Education Commission) of Pakistan. S.A.C.C. acknowledges support for the Scientific Employment Stimulus—Institutional Call (CEECINST/00102/2018).
Publisher Copyright:
© 2023 by the authors.
PY - 2023/7/15
Y1 - 2023/7/15
N2 - In this study, bimetallic nanoparticles (NPs) of silver (Ag) and zinc oxide (ZnO) were synthesized using Leptadenia pyrotechnica leaf extract for the first time. Monometallic NPs were also obtained for comparison. The characterization of the prepared NPs was carried out using various techniques, including UV-Visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The latter confirmed the crystalline nature and diameter of the monometallic and bimetallic NPs of Ag and ZnO. The SEM images of the prepared NPs revealed their different shapes. The biological activities of the NPs were evaluated concerning their antibacterial, antioxidant, and cytotoxic properties. The antibacterial activities were measured using the time-killing method. The results demonstrated that both the monometallic and bimetallic NPs inhibited the growth of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The antioxidant activities of the NPs were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and their cytotoxicity was checked using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results indicated that the controlled quantity of the monometallic and bimetallic NPs did not affect the viability of the cells. However, the decreased cell (L-929) viability suggested that the NPs could have anticancer properties. Furthermore, the photocatalytic degradation of methyl violet and 4-nitrophenol was investigated using the prepared Ag/ZnO NPs, examining the factors affecting the degradation process and conducting a kinetic and thermodynamic study. The prepared Ag/ZnO NPs demonstrated good photocatalytic degradation (88.9%) of the methyl violet (rate constant of 0.0183 min−1) in comparison to 4-nitrophenol (NPh), with a degradation rate of 81.37% and 0.0172 min−1, respectively. Overall, the bimetallic NPs showed superior antibacterial, antioxidant, cytotoxic, and photocatalytic properties compared to the monometallic NPs of Ag and ZnO.
AB - In this study, bimetallic nanoparticles (NPs) of silver (Ag) and zinc oxide (ZnO) were synthesized using Leptadenia pyrotechnica leaf extract for the first time. Monometallic NPs were also obtained for comparison. The characterization of the prepared NPs was carried out using various techniques, including UV-Visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The latter confirmed the crystalline nature and diameter of the monometallic and bimetallic NPs of Ag and ZnO. The SEM images of the prepared NPs revealed their different shapes. The biological activities of the NPs were evaluated concerning their antibacterial, antioxidant, and cytotoxic properties. The antibacterial activities were measured using the time-killing method. The results demonstrated that both the monometallic and bimetallic NPs inhibited the growth of Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The antioxidant activities of the NPs were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and their cytotoxicity was checked using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The results indicated that the controlled quantity of the monometallic and bimetallic NPs did not affect the viability of the cells. However, the decreased cell (L-929) viability suggested that the NPs could have anticancer properties. Furthermore, the photocatalytic degradation of methyl violet and 4-nitrophenol was investigated using the prepared Ag/ZnO NPs, examining the factors affecting the degradation process and conducting a kinetic and thermodynamic study. The prepared Ag/ZnO NPs demonstrated good photocatalytic degradation (88.9%) of the methyl violet (rate constant of 0.0183 min−1) in comparison to 4-nitrophenol (NPh), with a degradation rate of 81.37% and 0.0172 min−1, respectively. Overall, the bimetallic NPs showed superior antibacterial, antioxidant, cytotoxic, and photocatalytic properties compared to the monometallic NPs of Ag and ZnO.
KW - antibacterial
KW - antioxidant
KW - methyl violet
KW - photocatalytic degradation
KW - silver
KW - zinc oxide
UR - http://www.scopus.com/inward/record.url?scp=85166182447&partnerID=8YFLogxK
U2 - 10.3390/nano13142079
DO - 10.3390/nano13142079
M3 - Article
C2 - 37513090
AN - SCOPUS:85166182447
SN - 2079-4991
VL - 13
JO - Nanomaterials
JF - Nanomaterials
IS - 14
M1 - 2079
ER -