TY - JOUR
T1 - The allograft valve in heart transplantation and valve replacement
T2 - Genetic assessment of the origin of the cells by means of deoxyribonucleic acid profiles
AU - Melo, João Q.
AU - Monteiro, Carolino
AU - Neves, José
AU - Santos, Ricardo
AU - Martins, Ana
AU - Ramos, Sância
AU - Calta, Carol
AU - Matoso-Ferreira, António
AU - Viana, João
AU - Rueff, José
AU - Machado-Macedo, Manuel
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Assessment of the cellular origin of allograft valves is essential in comprehending their biologic behavior and is improving preparation methods. In this study we retrospectively analyzed 10 allografts obtained form patients who underwent valve replacement or heart transplantation. Histologic evaluation and deoxyribonucleic acid amplification by polymerase chain reaction technology with fluorescence labeled primers was performed on different parts of the valve leaflets. Automated analyses of the obtained amplifiers showed in the heart transplantation group the presence of receptor cells intersperses with native donor cells in three cases. Preliminary results for the valve replacement group are inconclusive as yet. ( J THORAC CARDIOVASC SURG 1995;109:218-23).
AB - Assessment of the cellular origin of allograft valves is essential in comprehending their biologic behavior and is improving preparation methods. In this study we retrospectively analyzed 10 allografts obtained form patients who underwent valve replacement or heart transplantation. Histologic evaluation and deoxyribonucleic acid amplification by polymerase chain reaction technology with fluorescence labeled primers was performed on different parts of the valve leaflets. Automated analyses of the obtained amplifiers showed in the heart transplantation group the presence of receptor cells intersperses with native donor cells in three cases. Preliminary results for the valve replacement group are inconclusive as yet. ( J THORAC CARDIOVASC SURG 1995;109:218-23).
UR - http://www.scopus.com/inward/record.url?scp=0028800689&partnerID=8YFLogxK
U2 - 10.1016/S0022-5223(95)70382-9
DO - 10.1016/S0022-5223(95)70382-9
M3 - Article
C2 - 7853875
AN - SCOPUS:0028800689
SN - 0022-5223
VL - 109
SP - 218
EP - 223
JO - The Journal of Thoracic and Cardiovascular Surgery
JF - The Journal of Thoracic and Cardiovascular Surgery
IS - 2
ER -